Independent pathways leading to apoptotic cell death, oxidative burst and defense gene expression in response to elicitin in tobacco cell suspension culture

Michiko Sasabe, Kasumi Takeuchi, Sophien Kamoun, Yuki Ichinose, Francine Govers, Kazuhiro Toyoda, Tomonori Shiraishi, Tetsuji Yamada

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

We characterized pharmacologically the hypersensitive cell death of tobacco BY-2 cells that followed treatments with Escherichia coli preparations of INF1, the major secreted elicitin of the late blight pathogen Phytophthora infestans. INF1 elicitin treatments resulted in fragmentation and 180 bp laddering of tobacco DNA as early as 3 h post-treatment. INF1 elicitin also induced rapid accumulation of H2O2 typical of oxidative burst, and the expression of defense genes such as phenylalanine ammonia-lyase (PAL) gene at 1 h and 3 h after elicitin treatment, respectively. To investigate the involvement of the oxidative burst and/or the expression of defense genes in the signal transduction pathways leading to hypersensitive cell death, we analyzed the effect of several chemical inhibitors of signal transduction pathways on the various responses. The results indicated that (a) the cell death required serine proteases, Ca2+ and protein kinases, (b) the oxidative burst was involved in Ca2+ and protein kinase mediated pathways, but elicitin-induced AOS was neither necessary nor sufficient for cell death and PAL gene expression, and (c) the signaling pathway of PAL gene expression required protein kinases. These results suggest that the three signal transduction pathways leading to cell death, oxidative burst and expression of defense genes branch in the early stages that follow elicitin recognition by tobacco cells.

Original languageEnglish
Pages (from-to)5005-5013
Number of pages9
JournalEuropean Journal of Biochemistry
Volume267
Issue number16
DOIs
Publication statusPublished - 2000

Fingerprint

Respiratory Burst
Tobacco
Cell death
Cell culture
Gene expression
Phenylalanine Ammonia-Lyase
Suspensions
Cell Death
Cell Culture Techniques
Signal transduction
Gene Expression
Genes
Protein Kinases
Signal Transduction
Phytophthora infestans
Protein-Serine-Threonine Kinases
Serine Proteases
Pathogens
Escherichia coli
DNA

Keywords

  • Defense response
  • DNA fragmentation
  • Hypersensitive cell death
  • Signal transduction
  • Tobacco BY-2

ASJC Scopus subject areas

  • Biochemistry

Cite this

Independent pathways leading to apoptotic cell death, oxidative burst and defense gene expression in response to elicitin in tobacco cell suspension culture. / Sasabe, Michiko; Takeuchi, Kasumi; Kamoun, Sophien; Ichinose, Yuki; Govers, Francine; Toyoda, Kazuhiro; Shiraishi, Tomonori; Yamada, Tetsuji.

In: European Journal of Biochemistry, Vol. 267, No. 16, 2000, p. 5005-5013.

Research output: Contribution to journalArticle

Sasabe, Michiko ; Takeuchi, Kasumi ; Kamoun, Sophien ; Ichinose, Yuki ; Govers, Francine ; Toyoda, Kazuhiro ; Shiraishi, Tomonori ; Yamada, Tetsuji. / Independent pathways leading to apoptotic cell death, oxidative burst and defense gene expression in response to elicitin in tobacco cell suspension culture. In: European Journal of Biochemistry. 2000 ; Vol. 267, No. 16. pp. 5005-5013.
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