Increasing participation of sclerostin in postnatal bone development, revealed by three-dimensional immunofluorescence morphometry

Takashi Watanabe, Yoshihiro Tamamura, Akiyoshi Hoshino, Yuji Makino, Hiroshi Kamioka, Teruo Amagasa, Akira Yamaguchi, Tadahiro Iimura

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Confocal immunofluorescence tiling imaging revealed the spatio-temporal distributions of osterix and sclerostin in femurs from 3-day-old, 2-week-old and 4-week-old rats to be reciprocally exclusive at the tissue level. Further quantitative three-dimensional immuno fluorescence morphometry demonstrated the increasing distribution of sclerostin in the osteocytic lacuno-canalicular system specifically in diaphysis, which paralleled the cooperative participation and depletion of osterix and β-catenin in adjacent periosteum cells. Treating MC3T3-E1 cells with BIO (a GSK3 inhibitor) induced the stabilization of β-catenin and nuclear translocation of osterix, and negatively regulated osteocalcin/BGLAP and Dmp1. These results collectively demonstrate that the increasing distribution of sclerostin in diaphyseal cortical bone appears to be involved in the attenuation of osterix and β-catenin in adjacent periosteum cells, thus possibly contributing to osteoblast maturation and reducing the osteoblast formation at this bone site. Our confocal microscopy-based imaging analyses provide a comprehensive and detailed view of the spatio-temporal distribution of sclerostin, β-catenin and osterix at the tissue to subcellular level in a coherent manner, and uncovered their spatio-temporal cooperation in postnatal bone development, thus providing evidence that they link skeletogenic growth and functional bone development.

Original languageEnglish
Pages (from-to)447-458
Number of pages12
JournalBone
Volume51
Issue number3
DOIs
Publication statusPublished - Sep 1 2012

Keywords

  • Imaging
  • Osteoblast
  • Osteocyte
  • Sclerostin

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Histology

Fingerprint Dive into the research topics of 'Increasing participation of sclerostin in postnatal bone development, revealed by three-dimensional immunofluorescence morphometry'. Together they form a unique fingerprint.

  • Cite this