Increases in mRNA levels for synapsin I but not synapsin II in the hippocampus of the rat kindling model of epilepsy

Kiyoshi Morimoto, Keiko Sato, Soichiro Sato, Shuji Suemaru, Toshiki Sato, Norihito Yamada, Toshiyuki Hayabara

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We studied brain synapsin I and II mRNA levels using the amygdala kindling model of epilepsy. There were significant increases in the synapsin I mRNA level in the granule cell layer of the hippocampal bilateral dentate gyrus. One to 8 h after seizures, the level in the dentate gyrus ipsilateral to stimulation increased by 44.2-73.2%, compared with the control level. Of the time points investigated, the greatest increase in expression was observed 8 h after the kindled seizures. Furthermore, the synapsin I mRNA levels in the dentate gyrus contralateral to stimulation increased by 28.0% and 51.1%, 2 and 8 h, respectively, after the kindled seizures. Expression of this mRNA, however, did not change significantly in other areas examined, including CA1, CA2, CA3 and the polymorphic layer of the hippocampus and the perirhinal and temporal cortices. Synapsin II mRNA levels did not change significantly in any of the regions studied for up to 24 h after the seizures and synapsin II was presumed to have little involvement in kindling. We considered the locally elevated synapsin I mRNA levels in the bilateral dentate gyrus associated with kindling indicate that excitatory changes occur in the synaptic circuit in which the dentate granule cells participate. Synapsin I may be involved in the presynaptic molecular mechanisms underlying the neuronal plasticity in kindling.

Original languageEnglish
Pages (from-to)229-235
Number of pages7
JournalSeizure
Volume7
Issue number3
DOIs
Publication statusPublished - 1998

Fingerprint

Synapsins
Epilepsy
Hippocampus
Messenger RNA
Dentate Gyrus
Seizures
Neuronal Plasticity
Temporal Lobe
Amygdala

Keywords

  • In situ hybridization
  • Kindling
  • Rats
  • Synapsin I
  • Synapsin II

ASJC Scopus subject areas

  • Clinical Neurology
  • Pediatrics, Perinatology, and Child Health
  • Neurology
  • Psychology(all)

Cite this

Increases in mRNA levels for synapsin I but not synapsin II in the hippocampus of the rat kindling model of epilepsy. / Morimoto, Kiyoshi; Sato, Keiko; Sato, Soichiro; Suemaru, Shuji; Sato, Toshiki; Yamada, Norihito; Hayabara, Toshiyuki.

In: Seizure, Vol. 7, No. 3, 1998, p. 229-235.

Research output: Contribution to journalArticle

Morimoto, Kiyoshi ; Sato, Keiko ; Sato, Soichiro ; Suemaru, Shuji ; Sato, Toshiki ; Yamada, Norihito ; Hayabara, Toshiyuki. / Increases in mRNA levels for synapsin I but not synapsin II in the hippocampus of the rat kindling model of epilepsy. In: Seizure. 1998 ; Vol. 7, No. 3. pp. 229-235.
@article{0b4bc22b95a9488b83e6b719a8b286b7,
title = "Increases in mRNA levels for synapsin I but not synapsin II in the hippocampus of the rat kindling model of epilepsy",
abstract = "We studied brain synapsin I and II mRNA levels using the amygdala kindling model of epilepsy. There were significant increases in the synapsin I mRNA level in the granule cell layer of the hippocampal bilateral dentate gyrus. One to 8 h after seizures, the level in the dentate gyrus ipsilateral to stimulation increased by 44.2-73.2{\%}, compared with the control level. Of the time points investigated, the greatest increase in expression was observed 8 h after the kindled seizures. Furthermore, the synapsin I mRNA levels in the dentate gyrus contralateral to stimulation increased by 28.0{\%} and 51.1{\%}, 2 and 8 h, respectively, after the kindled seizures. Expression of this mRNA, however, did not change significantly in other areas examined, including CA1, CA2, CA3 and the polymorphic layer of the hippocampus and the perirhinal and temporal cortices. Synapsin II mRNA levels did not change significantly in any of the regions studied for up to 24 h after the seizures and synapsin II was presumed to have little involvement in kindling. We considered the locally elevated synapsin I mRNA levels in the bilateral dentate gyrus associated with kindling indicate that excitatory changes occur in the synaptic circuit in which the dentate granule cells participate. Synapsin I may be involved in the presynaptic molecular mechanisms underlying the neuronal plasticity in kindling.",
keywords = "In situ hybridization, Kindling, Rats, Synapsin I, Synapsin II",
author = "Kiyoshi Morimoto and Keiko Sato and Soichiro Sato and Shuji Suemaru and Toshiki Sato and Norihito Yamada and Toshiyuki Hayabara",
year = "1998",
doi = "10.1016/S1059-1311(98)80041-1",
language = "English",
volume = "7",
pages = "229--235",
journal = "Seizure : the journal of the British Epilepsy Association",
issn = "1059-1311",
publisher = "W.B. Saunders Ltd",
number = "3",

}

TY - JOUR

T1 - Increases in mRNA levels for synapsin I but not synapsin II in the hippocampus of the rat kindling model of epilepsy

AU - Morimoto, Kiyoshi

AU - Sato, Keiko

AU - Sato, Soichiro

AU - Suemaru, Shuji

AU - Sato, Toshiki

AU - Yamada, Norihito

AU - Hayabara, Toshiyuki

PY - 1998

Y1 - 1998

N2 - We studied brain synapsin I and II mRNA levels using the amygdala kindling model of epilepsy. There were significant increases in the synapsin I mRNA level in the granule cell layer of the hippocampal bilateral dentate gyrus. One to 8 h after seizures, the level in the dentate gyrus ipsilateral to stimulation increased by 44.2-73.2%, compared with the control level. Of the time points investigated, the greatest increase in expression was observed 8 h after the kindled seizures. Furthermore, the synapsin I mRNA levels in the dentate gyrus contralateral to stimulation increased by 28.0% and 51.1%, 2 and 8 h, respectively, after the kindled seizures. Expression of this mRNA, however, did not change significantly in other areas examined, including CA1, CA2, CA3 and the polymorphic layer of the hippocampus and the perirhinal and temporal cortices. Synapsin II mRNA levels did not change significantly in any of the regions studied for up to 24 h after the seizures and synapsin II was presumed to have little involvement in kindling. We considered the locally elevated synapsin I mRNA levels in the bilateral dentate gyrus associated with kindling indicate that excitatory changes occur in the synaptic circuit in which the dentate granule cells participate. Synapsin I may be involved in the presynaptic molecular mechanisms underlying the neuronal plasticity in kindling.

AB - We studied brain synapsin I and II mRNA levels using the amygdala kindling model of epilepsy. There were significant increases in the synapsin I mRNA level in the granule cell layer of the hippocampal bilateral dentate gyrus. One to 8 h after seizures, the level in the dentate gyrus ipsilateral to stimulation increased by 44.2-73.2%, compared with the control level. Of the time points investigated, the greatest increase in expression was observed 8 h after the kindled seizures. Furthermore, the synapsin I mRNA levels in the dentate gyrus contralateral to stimulation increased by 28.0% and 51.1%, 2 and 8 h, respectively, after the kindled seizures. Expression of this mRNA, however, did not change significantly in other areas examined, including CA1, CA2, CA3 and the polymorphic layer of the hippocampus and the perirhinal and temporal cortices. Synapsin II mRNA levels did not change significantly in any of the regions studied for up to 24 h after the seizures and synapsin II was presumed to have little involvement in kindling. We considered the locally elevated synapsin I mRNA levels in the bilateral dentate gyrus associated with kindling indicate that excitatory changes occur in the synaptic circuit in which the dentate granule cells participate. Synapsin I may be involved in the presynaptic molecular mechanisms underlying the neuronal plasticity in kindling.

KW - In situ hybridization

KW - Kindling

KW - Rats

KW - Synapsin I

KW - Synapsin II

UR - http://www.scopus.com/inward/record.url?scp=0031927385&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031927385&partnerID=8YFLogxK

U2 - 10.1016/S1059-1311(98)80041-1

DO - 10.1016/S1059-1311(98)80041-1

M3 - Article

C2 - 9700837

AN - SCOPUS:0031927385

VL - 7

SP - 229

EP - 235

JO - Seizure : the journal of the British Epilepsy Association

JF - Seizure : the journal of the British Epilepsy Association

SN - 1059-1311

IS - 3

ER -