Increased plasma GlyCAM-1, a mouse L-selectin ligand, in response to an inflammatory stimulus

Takayasu Suguri, Akio Kikuta, Hiromi Iwagaki, Tadashi Yoshino, Noriaki Tanaka, Kunzo Orita

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

GlyCAM-1 (glycosylation-dependent cell adhesion molecule-1) is one of the sialomucin-like ligands for L-selectin, which is a member of the selectin family and mediates initial adhesion of leukocytes to specialized high endothelial venules in lymph nodes and venules at sites of inflammation. GlyCAM-1, lacking a transmembrane domain, is supposed to be secreted into the blood. To understand the functional role of secreted GlyCAM-1, we performed sandwich enzyme-linked immunosorbent assay to measure GlyCAM-1 plasma levels after inflammatory stimulus. BALB/c mice were injected with complete Freund's adjuvant (CFA) in the hind footpads; serum levels of GlyCAM-1 and L-selectin bound to GlyCAM-1 and several inflammatory cytokines, including interleukin-6 (IL-6), were measured at various intervals. IL-6 showed a significant increase 3 h after CFA stimulation. GlyCAM-1 was increased at 3 h, reached peak levels at 12 h, and gradually decreased thereafter. Levels of L-selectin bound to the plasma GlyCAM-1 changed over a similar time course, reached peak at 12 h after, and then began to decrease. The binding of L-selectin to plasma GlyCAM-1 was completely eliminated with the presence of ethyleneglycol-bis (β-aminoethylether)-N,N'-tetraacetic acid, showing the calcium dependency of this binding. These findings show that GlyCAM-1 release is enhanced by inflammatory stimulation and also suggest that released plasma GlyCAM-1 may trap, at least in part, soluble L-selectin shed from stimulated leukocytes to neutralize each other.

Original languageEnglish
Pages (from-to)593-597
Number of pages5
JournalJournal of Leukocyte Biology
Volume60
Issue number5
Publication statusPublished - Nov 1996

Fingerprint

L-Selectin
Ligands
Venules
Freund's Adjuvant
Interleukin-6
Leukocytes
sulfated glycoprotein p50
Sialomucins
Selectins
Lymph Nodes
Enzyme-Linked Immunosorbent Assay

Keywords

  • Complete Freund's adjuvant
  • Endothelial venules
  • Inflammation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Increased plasma GlyCAM-1, a mouse L-selectin ligand, in response to an inflammatory stimulus. / Suguri, Takayasu; Kikuta, Akio; Iwagaki, Hiromi; Yoshino, Tadashi; Tanaka, Noriaki; Orita, Kunzo.

In: Journal of Leukocyte Biology, Vol. 60, No. 5, 11.1996, p. 593-597.

Research output: Contribution to journalArticle

Suguri, T, Kikuta, A, Iwagaki, H, Yoshino, T, Tanaka, N & Orita, K 1996, 'Increased plasma GlyCAM-1, a mouse L-selectin ligand, in response to an inflammatory stimulus', Journal of Leukocyte Biology, vol. 60, no. 5, pp. 593-597.
Suguri, Takayasu ; Kikuta, Akio ; Iwagaki, Hiromi ; Yoshino, Tadashi ; Tanaka, Noriaki ; Orita, Kunzo. / Increased plasma GlyCAM-1, a mouse L-selectin ligand, in response to an inflammatory stimulus. In: Journal of Leukocyte Biology. 1996 ; Vol. 60, No. 5. pp. 593-597.
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