Increased passive stiffness of cardiomyocytes in the transverse direction and residual actin and myosin cross-bridge formation in hypertrophied rat hearts induced by chronic β-adrenergic stimulation

Wakako Sumita Yoshikawa, Kazufumi Nakamura, Daiji Miura, Juichiro Shimizu, Ken Hashimoto, Noriyuki Kataoka, Hiroko Toyota, Hiroshi Okuyama, Toru Miyoshi, Hiroshi Morita, Kengo Fukushima Kusano, Tatsuhito Matsuo, Miyako Takaki, Fumihiko Kajiya, Naoto Yagi, Tohru Ohe, Hiroshi Itoh

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background:Left ventricular (LV) hypertrophy is often present in patients with diastolic heart failure. However, stiffness of hypertrophied cardiomyocytes in the transverse direction has not been fully elucidated. The aim of this study was to assess passive cardiomyocyte stiffness of hypertrophied hearts in the transverse direction and the influence of actin-myosin cross-bridge formation on the stiffness. Methods and Results:Wistar rats received a vehicle (control) or isoproterenol (ISO) subcutaneously. After 7 days, compared with the controls, ISO administration had significantly increased heart weight and LV wall thickness and had decreased peak early annular relaxation velocity (e') assessed by echocardiography. Elastic modulus of living cardiomyocytes in the transverse direction assessed by an atomic force microscope was significantly higher in the ISO group than in controls. We added butanedione monoxime (BDM), an inhibitor of actin-myosin interaction, and blebbistatin, a specific myosin II inhibitor, to the medium. BDM and blebbistatin significantly reduced the elastic modulus of cardiomyocytes in the ISO group. X-ray diffraction analysis showed that the reflection intensity ratio (I(1,0)/I(1,1)) at diastole was not different before and after treatment with BDM, which induces complete relaxation, in control hearts, but that I(1,0)/I(1,1) was significantly increased after BDM treatment in the ISO group, indicating residual cross-bridge formation in hypertrophied hearts. Conclusions:Passive cardiomyocyte stiffness in the transverse direction is increased in hearts with ISO-induced hypertrophy and this is caused by residual actin-myosin cross-bridge formation.

Original languageEnglish
Pages (from-to)741-748
Number of pages8
JournalCirculation Journal
Volume77
Issue number3
DOIs
Publication statusPublished - 2013

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Myosins
Isoproterenol
Cardiac Myocytes
Adrenergic Agents
Actins
Elastic Modulus
Diastolic Heart Failure
Myosin Type II
Diastole
Left Ventricular Hypertrophy
X-Ray Diffraction
Hypertrophy
Echocardiography
Direction compound
Wistar Rats
Weights and Measures
Control Groups
diacetylmonoxime
Therapeutics

Keywords

  • Diastolic dysfunction
  • Heart failure
  • Myocytes
  • Stiffness

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Increased passive stiffness of cardiomyocytes in the transverse direction and residual actin and myosin cross-bridge formation in hypertrophied rat hearts induced by chronic β-adrenergic stimulation. / Yoshikawa, Wakako Sumita; Nakamura, Kazufumi; Miura, Daiji; Shimizu, Juichiro; Hashimoto, Ken; Kataoka, Noriyuki; Toyota, Hiroko; Okuyama, Hiroshi; Miyoshi, Toru; Morita, Hiroshi; Kusano, Kengo Fukushima; Matsuo, Tatsuhito; Takaki, Miyako; Kajiya, Fumihiko; Yagi, Naoto; Ohe, Tohru; Itoh, Hiroshi.

In: Circulation Journal, Vol. 77, No. 3, 2013, p. 741-748.

Research output: Contribution to journalArticle

Yoshikawa, Wakako Sumita ; Nakamura, Kazufumi ; Miura, Daiji ; Shimizu, Juichiro ; Hashimoto, Ken ; Kataoka, Noriyuki ; Toyota, Hiroko ; Okuyama, Hiroshi ; Miyoshi, Toru ; Morita, Hiroshi ; Kusano, Kengo Fukushima ; Matsuo, Tatsuhito ; Takaki, Miyako ; Kajiya, Fumihiko ; Yagi, Naoto ; Ohe, Tohru ; Itoh, Hiroshi. / Increased passive stiffness of cardiomyocytes in the transverse direction and residual actin and myosin cross-bridge formation in hypertrophied rat hearts induced by chronic β-adrenergic stimulation. In: Circulation Journal. 2013 ; Vol. 77, No. 3. pp. 741-748.
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abstract = "Background:Left ventricular (LV) hypertrophy is often present in patients with diastolic heart failure. However, stiffness of hypertrophied cardiomyocytes in the transverse direction has not been fully elucidated. The aim of this study was to assess passive cardiomyocyte stiffness of hypertrophied hearts in the transverse direction and the influence of actin-myosin cross-bridge formation on the stiffness. Methods and Results:Wistar rats received a vehicle (control) or isoproterenol (ISO) subcutaneously. After 7 days, compared with the controls, ISO administration had significantly increased heart weight and LV wall thickness and had decreased peak early annular relaxation velocity (e') assessed by echocardiography. Elastic modulus of living cardiomyocytes in the transverse direction assessed by an atomic force microscope was significantly higher in the ISO group than in controls. We added butanedione monoxime (BDM), an inhibitor of actin-myosin interaction, and blebbistatin, a specific myosin II inhibitor, to the medium. BDM and blebbistatin significantly reduced the elastic modulus of cardiomyocytes in the ISO group. X-ray diffraction analysis showed that the reflection intensity ratio (I(1,0)/I(1,1)) at diastole was not different before and after treatment with BDM, which induces complete relaxation, in control hearts, but that I(1,0)/I(1,1) was significantly increased after BDM treatment in the ISO group, indicating residual cross-bridge formation in hypertrophied hearts. Conclusions:Passive cardiomyocyte stiffness in the transverse direction is increased in hearts with ISO-induced hypertrophy and this is caused by residual actin-myosin cross-bridge formation.",
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TY - JOUR

T1 - Increased passive stiffness of cardiomyocytes in the transverse direction and residual actin and myosin cross-bridge formation in hypertrophied rat hearts induced by chronic β-adrenergic stimulation

AU - Yoshikawa, Wakako Sumita

AU - Nakamura, Kazufumi

AU - Miura, Daiji

AU - Shimizu, Juichiro

AU - Hashimoto, Ken

AU - Kataoka, Noriyuki

AU - Toyota, Hiroko

AU - Okuyama, Hiroshi

AU - Miyoshi, Toru

AU - Morita, Hiroshi

AU - Kusano, Kengo Fukushima

AU - Matsuo, Tatsuhito

AU - Takaki, Miyako

AU - Kajiya, Fumihiko

AU - Yagi, Naoto

AU - Ohe, Tohru

AU - Itoh, Hiroshi

PY - 2013

Y1 - 2013

N2 - Background:Left ventricular (LV) hypertrophy is often present in patients with diastolic heart failure. However, stiffness of hypertrophied cardiomyocytes in the transverse direction has not been fully elucidated. The aim of this study was to assess passive cardiomyocyte stiffness of hypertrophied hearts in the transverse direction and the influence of actin-myosin cross-bridge formation on the stiffness. Methods and Results:Wistar rats received a vehicle (control) or isoproterenol (ISO) subcutaneously. After 7 days, compared with the controls, ISO administration had significantly increased heart weight and LV wall thickness and had decreased peak early annular relaxation velocity (e') assessed by echocardiography. Elastic modulus of living cardiomyocytes in the transverse direction assessed by an atomic force microscope was significantly higher in the ISO group than in controls. We added butanedione monoxime (BDM), an inhibitor of actin-myosin interaction, and blebbistatin, a specific myosin II inhibitor, to the medium. BDM and blebbistatin significantly reduced the elastic modulus of cardiomyocytes in the ISO group. X-ray diffraction analysis showed that the reflection intensity ratio (I(1,0)/I(1,1)) at diastole was not different before and after treatment with BDM, which induces complete relaxation, in control hearts, but that I(1,0)/I(1,1) was significantly increased after BDM treatment in the ISO group, indicating residual cross-bridge formation in hypertrophied hearts. Conclusions:Passive cardiomyocyte stiffness in the transverse direction is increased in hearts with ISO-induced hypertrophy and this is caused by residual actin-myosin cross-bridge formation.

AB - Background:Left ventricular (LV) hypertrophy is often present in patients with diastolic heart failure. However, stiffness of hypertrophied cardiomyocytes in the transverse direction has not been fully elucidated. The aim of this study was to assess passive cardiomyocyte stiffness of hypertrophied hearts in the transverse direction and the influence of actin-myosin cross-bridge formation on the stiffness. Methods and Results:Wistar rats received a vehicle (control) or isoproterenol (ISO) subcutaneously. After 7 days, compared with the controls, ISO administration had significantly increased heart weight and LV wall thickness and had decreased peak early annular relaxation velocity (e') assessed by echocardiography. Elastic modulus of living cardiomyocytes in the transverse direction assessed by an atomic force microscope was significantly higher in the ISO group than in controls. We added butanedione monoxime (BDM), an inhibitor of actin-myosin interaction, and blebbistatin, a specific myosin II inhibitor, to the medium. BDM and blebbistatin significantly reduced the elastic modulus of cardiomyocytes in the ISO group. X-ray diffraction analysis showed that the reflection intensity ratio (I(1,0)/I(1,1)) at diastole was not different before and after treatment with BDM, which induces complete relaxation, in control hearts, but that I(1,0)/I(1,1) was significantly increased after BDM treatment in the ISO group, indicating residual cross-bridge formation in hypertrophied hearts. Conclusions:Passive cardiomyocyte stiffness in the transverse direction is increased in hearts with ISO-induced hypertrophy and this is caused by residual actin-myosin cross-bridge formation.

KW - Diastolic dysfunction

KW - Heart failure

KW - Myocytes

KW - Stiffness

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U2 - 10.1253/circj.CJ-12-0779

DO - 10.1253/circj.CJ-12-0779

M3 - Article

VL - 77

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EP - 748

JO - Circulation Journal

JF - Circulation Journal

SN - 1346-9843

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