Increased oxidative stress in rats with chronic nitric oxide depletion: Measurement of urinary 8-hydroxy-2'-deoxyguanosine excretion

Hirokazu Tsukahara, Masahiro Hiraoka, Ritsuyo Kobata, Ikue Hata, Yusei Ohshima, Mi Zu Jiang, Eisei Noiri, Mitsufumi Mayumi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been reported to serve as a sensitive biomarker of oxidative stress. We examined the effect of chronic blockade of nitric oxide (NO) on urinary excretion of 8-OHdG in rats. Two types of NO synthase inhibitor were used: N(G)-nitro-Larginine methyl ester (L-NAME) as a non-selective inhibitor and aminoguanidine (AG) as aselective inhibitor of the inducible isoform. Oral administration of L-NAME (20, 50 and 80 mg/dl of drinking water), but not AG (400 mg/dl), for 4 weeks induced systemic hypertension and a significant reduction in urinary excretion of NO2-/NO3-. Rats treated with L-NAME also showed a significant increase in urinary 8-OHdG excretion compared with the control animals. The effects of L-NAME (50 mg/dl) on blood pressure and urinary excretion of NO2-/NO3- and 8-OHdG were restored by a large dose of L-arginine (2.0 g/dl). Chronic AG administration did not significantly alter urinary 8-OHdG excretion. On combining all the data, there was a significant negative correlation between urinary NO2-/NO3- and 8-OHdG. These observations suggest the importance of constitutive NO synthase activity in the maintenance of oxidant buffering capacity in rats. Oral administration of L-NAME may serve as a model of hypertension due to chronic NO deficiency with increased oxidative stress.

Original languageEnglish
Pages (from-to)23-28
Number of pages6
JournalRedox Report
Volume5
Issue number1
Publication statusPublished - 2000
Externally publishedYes

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Oxidative stress
NG-Nitroarginine Methyl Ester
Rats
Nitric Oxide
Oxidative Stress
Nitric Oxide Synthase
Oral Administration
Hypertension
Blood pressure
Biomarkers
Oxidants
Drinking Water
Arginine
Protein Isoforms
Esters
Animals
Maintenance
8-oxo-7-hydrodeoxyguanosine
Blood Pressure
pimagedine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Increased oxidative stress in rats with chronic nitric oxide depletion : Measurement of urinary 8-hydroxy-2'-deoxyguanosine excretion. / Tsukahara, Hirokazu; Hiraoka, Masahiro; Kobata, Ritsuyo; Hata, Ikue; Ohshima, Yusei; Jiang, Mi Zu; Noiri, Eisei; Mayumi, Mitsufumi.

In: Redox Report, Vol. 5, No. 1, 2000, p. 23-28.

Research output: Contribution to journalArticle

Tsukahara, H, Hiraoka, M, Kobata, R, Hata, I, Ohshima, Y, Jiang, MZ, Noiri, E & Mayumi, M 2000, 'Increased oxidative stress in rats with chronic nitric oxide depletion: Measurement of urinary 8-hydroxy-2'-deoxyguanosine excretion', Redox Report, vol. 5, no. 1, pp. 23-28.
Tsukahara, Hirokazu ; Hiraoka, Masahiro ; Kobata, Ritsuyo ; Hata, Ikue ; Ohshima, Yusei ; Jiang, Mi Zu ; Noiri, Eisei ; Mayumi, Mitsufumi. / Increased oxidative stress in rats with chronic nitric oxide depletion : Measurement of urinary 8-hydroxy-2'-deoxyguanosine excretion. In: Redox Report. 2000 ; Vol. 5, No. 1. pp. 23-28.
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