Increased osteocyte apoptosis during the development of femoral head osteonecrosis in spontaneously hypertensive rats

Motoi Shibahara, Keiichiro Nishida, Hiroshi Asahara, Takeshi Yoshikawa, Shigeru Mitani, Yoichi Kondo, Hajime Inoue

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We investigated the presence of osteocyte apoptosis in the necrotic trabeculae of the femoral head of spontaneously hypertensive rat (SHR) using the in situ nick end labeling (TUNEL) method and transmission electron microscopy. The occurrence of osteonecrosis and ossification disturbance was significantly higher in SHR compared with Wistar Kyoto (WKY) rats, and Wistar (WT) rats used as control animals (P <0.01). A high population of TUNEL positive osteocytes was detected mainly in 10- and 15-week-old SHRs. Sectioned examination of the femoral head of SHRs and WKY rats by electron microscopy revealed apoptotic cell appearances such as aggregation of chromatin particles and lipid formation. In contrast, a positive reaction was significantly lower in osteocytes in the femoral heads of WT rats (P <0.01). Our results indicate that apoptosis forms an important component of the global pathologic process affecting the femoral head of SHR, which leads to osteonecrosis in this region.

Original languageEnglish
Pages (from-to)67-74
Number of pages8
JournalActa Medica Okayama
Volume54
Issue number2
Publication statusPublished - 2000

Fingerprint

Osteocytes
Osteonecrosis
Inbred SHR Rats
Thigh
Rats
Apoptosis
Inbred WKY Rats
In Situ Nick-End Labeling
Wistar Rats
Labeling
Pathologic Processes
Transmission Electron Microscopy
Osteogenesis
Chromatin
Electron Microscopy
Lipids
Electron microscopy
Animals
Population
Agglomeration

Keywords

  • Apoptosis
  • Osteonecrosis of the femoral head
  • Spontaneously hypertensive rat

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Shibahara, M., Nishida, K., Asahara, H., Yoshikawa, T., Mitani, S., Kondo, Y., & Inoue, H. (2000). Increased osteocyte apoptosis during the development of femoral head osteonecrosis in spontaneously hypertensive rats. Acta Medica Okayama, 54(2), 67-74.

Increased osteocyte apoptosis during the development of femoral head osteonecrosis in spontaneously hypertensive rats. / Shibahara, Motoi; Nishida, Keiichiro; Asahara, Hiroshi; Yoshikawa, Takeshi; Mitani, Shigeru; Kondo, Yoichi; Inoue, Hajime.

In: Acta Medica Okayama, Vol. 54, No. 2, 2000, p. 67-74.

Research output: Contribution to journalArticle

Shibahara, M, Nishida, K, Asahara, H, Yoshikawa, T, Mitani, S, Kondo, Y & Inoue, H 2000, 'Increased osteocyte apoptosis during the development of femoral head osteonecrosis in spontaneously hypertensive rats', Acta Medica Okayama, vol. 54, no. 2, pp. 67-74.
Shibahara, Motoi ; Nishida, Keiichiro ; Asahara, Hiroshi ; Yoshikawa, Takeshi ; Mitani, Shigeru ; Kondo, Yoichi ; Inoue, Hajime. / Increased osteocyte apoptosis during the development of femoral head osteonecrosis in spontaneously hypertensive rats. In: Acta Medica Okayama. 2000 ; Vol. 54, No. 2. pp. 67-74.
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