Increased level of heme oxygenase-1 in rheumatoid arthritis synovial fluid

Ai Kitamura, Keiichiro Nishida, Takamitsu Komiyama, Hideyuki Doi, Yasutaka Kadota, Aki Yoshida, Toshihumi Ozaki

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We investigated the expression and localization of heme oxygenase-1 (HO-1) in synovial fluid and synovial tissue, and examined the stimulation of HO-1 production in rheumatoid synovial fibroblasts (RASFs). Synovial fluid samples were obtained from knee joints of 20 rheumatoid arthritis (RA) and 20 osteoarthritis (OA) patients, and concentration of HO-1 and matrix metalloproteinase-3 (MMP-3) were measured by enzyme-linked immunosorbent assay (ELISA). Synovial tissues obtained from RA or OA patients during total knee arthroplasty (TKA) were used for immunohistochemical analysis of HO-1. HO-1 production by RASFs in response to various cytokines was examined by ELISA. HO-1 levels in synovial fluid were higher in the RA group than in the OA group with significant difference (P

Original languageEnglish
Pages (from-to)150-157
Number of pages8
JournalModern Rheumatology
Issue number2
Publication statusPublished - Apr 2011



  • C-reactive protein (CRP)
  • Heme oxygenase-1 (HO-1)
  • Joint fluid
  • Matrix metalloproteinase (MMP)-3
  • Rheumatoid arthritis

ASJC Scopus subject areas

  • Rheumatology

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