Increased hydrostatic pressure induces nuclear translocation of DAF-16/FOXO in C. elegans

Naoshi Watanabe, Masatoshi Morimatsu, Ayano Fujita, Mika Teranishi, Surabhi Sudevan, Masaru Watanabe, Hiroaki Iwasa, Yutaka Hata, Hiroyuki Kagi, Masayoshi Nishiyama, Keiji Naruse, Atsushi Higashitani

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Abstract

Mechanical stimulation is well known to be important for maintaining tissue and organ homeostasis. Here, we found that hydrostatic pressure induced nuclear translocation of a forkhead box O (FOXO) transcription factor DAF-16, in C. elegans within minutes, whereas the removal of this pressure resulted in immediate export of DAF-16 to the cytoplasm. We also monitored DAF-16-dependent transcriptional changes by exposure to 1 MPa pressure for 5 min, and found significant changes in collagen and other genes in a DAF-16 dependent manner. Lifespan was markedly prolonged with exposure to cyclic pressure treatment (1 MPa once a day for 5 min from L1 larvae until death). Furthermore, age-dependent decline in locomotor activity was suppressed by the treatment. In contrast, the nuclear translocation of the yes-associated protein YAP-1 was not induced under the same pressure conditions. Thus, moderate hydrostatic pressure improves ageing progression through activation of DAF-16/FOXO in C. elegans.

Original languageEnglish
Pages (from-to)853-858
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume523
Issue number4
DOIs
Publication statusPublished - Mar 19 2020

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Keywords

  • Ageing
  • Longevity
  • Mechanical stimulation
  • Mega pascal
  • Transcription factor

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Watanabe, N., Morimatsu, M., Fujita, A., Teranishi, M., Sudevan, S., Watanabe, M., Iwasa, H., Hata, Y., Kagi, H., Nishiyama, M., Naruse, K., & Higashitani, A. (2020). Increased hydrostatic pressure induces nuclear translocation of DAF-16/FOXO in C. elegans. Biochemical and Biophysical Research Communications, 523(4), 853-858. https://doi.org/10.1016/j.bbrc.2020.01.047