Increased hydrostatic pressure induces nuclear translocation of DAF-16/FOXO in C. elegans

Naoshi Watanabe, Masatoshi Morimatsu, Ayano Fujita, Mika Teranishi, Surabhi Sudevan, Masaru Watanabe, Hiroaki Iwasa, Yutaka Hata, Hiroyuki Kagi, Masayoshi Nishiyama, Keiji Naruse, Atsushi Higashitani

Research output: Contribution to journalArticle

Abstract

Mechanical stimulation is well known to be important for maintaining tissue and organ homeostasis. Here, we found that hydrostatic pressure induced nuclear translocation of a forkhead box O (FOXO) transcription factor DAF-16, in C. elegans within minutes, whereas the removal of this pressure resulted in immediate export of DAF-16 to the cytoplasm. We also monitored DAF-16-dependent transcriptional changes by exposure to 1 MPa pressure for 5 min, and found significant changes in collagen and other genes in a DAF-16 dependent manner. Lifespan was markedly prolonged with exposure to cyclic pressure treatment (1 MPa once a day for 5 min from L1 larvae until death). Furthermore, age-dependent decline in locomotor activity was suppressed by the treatment. In contrast, the nuclear translocation of the yes-associated protein YAP-1 was not induced under the same pressure conditions. Thus, moderate hydrostatic pressure improves ageing progression through activation of DAF-16/FOXO in C. elegans.

Original languageEnglish
JournalBiochemical and Biophysical Research Communications
DOIs
Publication statusAccepted/In press - Jan 1 2020

Fingerprint

Hydrostatic Pressure
Hydrostatic pressure
Pressure
Forkhead Transcription Factors
Locomotion
Larva
Cytoplasm
Homeostasis
Transcription Factors
Collagen
Genes
Aging of materials
Chemical activation
Tissue
Proteins

Keywords

  • Ageing
  • Longevity
  • Mechanical stimulation
  • Mega pascal
  • Transcription factor

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Increased hydrostatic pressure induces nuclear translocation of DAF-16/FOXO in C. elegans. / Watanabe, Naoshi; Morimatsu, Masatoshi; Fujita, Ayano; Teranishi, Mika; Sudevan, Surabhi; Watanabe, Masaru; Iwasa, Hiroaki; Hata, Yutaka; Kagi, Hiroyuki; Nishiyama, Masayoshi; Naruse, Keiji; Higashitani, Atsushi.

In: Biochemical and Biophysical Research Communications, 01.01.2020.

Research output: Contribution to journalArticle

Watanabe, Naoshi ; Morimatsu, Masatoshi ; Fujita, Ayano ; Teranishi, Mika ; Sudevan, Surabhi ; Watanabe, Masaru ; Iwasa, Hiroaki ; Hata, Yutaka ; Kagi, Hiroyuki ; Nishiyama, Masayoshi ; Naruse, Keiji ; Higashitani, Atsushi. / Increased hydrostatic pressure induces nuclear translocation of DAF-16/FOXO in C. elegans. In: Biochemical and Biophysical Research Communications. 2020.
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AU - Sudevan, Surabhi

AU - Watanabe, Masaru

AU - Iwasa, Hiroaki

AU - Hata, Yutaka

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AU - Nishiyama, Masayoshi

AU - Naruse, Keiji

AU - Higashitani, Atsushi

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