Increased gene expression of insulin-like growth factor-i receptor in experimental diabetic rat glomeruli

Hikaru Sugimoto, Kenichi Shikata, Hirofumi Makino, Kosuke Ota, Zensuke Ota

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Renal hypertrophy is a characteristic and early manifestation of diabetes in humans and experimental animals. We examined the precise distribution of insulin-like growth factor-I (IGF-I) mRNA and IGF-I receptor mRNA in the experimental diabetic rat kidney using a nonradioactive in situ hybridization technique. No significant difference in the distribution of IGF-I mRNA was found between the diabetic and control rats. IGF-I mRNA-positive cells were found in the collecting ducts and in scattered single cells in the distal tubules. The number of IGF-I mRNA-positive cells was very low in the glomeruli. Expression of IGF-I receptor mRNA was rarely seen in the glomeruli of control rats. IGF-I receptor mRNA was detected after induction of diabetes in glomerular mesangial, visceral epithelial, and parietal epithelial cells. The number of IGF-I receptor mRNA-positive cells in a glomerulus increased significantly, peaking at 4 weeks as compared with the control rats. Overexpression of IGF-I receptor in glomerular cells, especially mesangial and visceral epithelial cells, may contribute to glomerular hypertrophy in diabetic nephropathy.

Original languageEnglish
Pages (from-to)648-653
Number of pages6
JournalNephron
Volume72
Issue number4
DOIs
Publication statusPublished - Jan 1 1996

Keywords

  • Diabetic nephropathy
  • Hybridization
  • IGF-I receptor
  • In situ
  • Insulin-like growth factor-I
  • Renal hypertrophy

ASJC Scopus subject areas

  • Physiology
  • Nephrology
  • Physiology (medical)
  • Urology

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