Increased expression of receptor for advanced glycation end products by synovial tissue macrophages in rheumatoid arthritis

Katsue Sunahori, Masahiro Yamamura, Jiro Yamana, Koji Takasugi, Masanori Kawashima, Hirofumi Makino

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Objective. The accumulation of advanced glycation end products (AGEs), S100A12, and high mobility group box chromosomal protein 1 has been associated with joint inflammation in rheumatoid arthritis (RA). This study was undertaken to determine the induction of the receptor for these proteins, termed receptor for AGEs (RAGE), in synovial tissue (ST) macrophages from RA patients. Methods. RAGE and CD68 expression in ST were determined by 2-color immunofluorescence labeling. Cell surface and messenger RNA (mRNA) expression of RAGE were examined by flow cytometry and reverse transcriptase-polymerase chain reaction (PCR) or real-time PCR, respectively. Results. CD68+ lining macrophages, like the vasculature, expressed high levels of RAGE in inflamed ST from RA patients. RAGE mRNA expression was significantly higher in RA ST than in ST from patients with osteoarthritis. RAGE mRNA levels were significantly higher in ST macrophages and normal endothelial cells than in ST CD4+ T cells and synovial fibroblasts stimulated with tumor necrosis factor α and interleukin-l (IL-1β). Cell surface RAGE was highly induced on normal monocytes after a 24-hour incubation with a 20% concentration of RA ST cell culture supernatants. RAGE mRNA expression in adherent monocytes was augmented by various cytokines, most potently by IL-1β. Conclusion. These results indicate that RAGE overexpression in lining macrophages may be induced, at least in part, by cytokines such as IL-1, leading to the amplification of inflammatory responses mediated by RAGE ligands that are abundant in RA joints.

Original languageEnglish
Pages (from-to)97-104
Number of pages8
JournalArthritis and Rheumatism
Volume54
Issue number1
DOIs
Publication statusPublished - Jan 1 2006

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Rheumatoid Arthritis
Macrophages
Interleukin-1
Messenger RNA
Monocytes
Joints
High Mobility Group Proteins
Cytokines
HMGB1 Protein
Advanced Glycosylation End Product-Specific Receptor
Advanced Glycosylation End Products
Interleukins
Cell Surface Receptors
Reverse Transcriptase Polymerase Chain Reaction
Osteoarthritis
Fluorescent Antibody Technique
Real-Time Polymerase Chain Reaction
Flow Cytometry
Endothelial Cells
Color

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Increased expression of receptor for advanced glycation end products by synovial tissue macrophages in rheumatoid arthritis. / Sunahori, Katsue; Yamamura, Masahiro; Yamana, Jiro; Takasugi, Koji; Kawashima, Masanori; Makino, Hirofumi.

In: Arthritis and Rheumatism, Vol. 54, No. 1, 01.01.2006, p. 97-104.

Research output: Contribution to journalArticle

Sunahori, Katsue ; Yamamura, Masahiro ; Yamana, Jiro ; Takasugi, Koji ; Kawashima, Masanori ; Makino, Hirofumi. / Increased expression of receptor for advanced glycation end products by synovial tissue macrophages in rheumatoid arthritis. In: Arthritis and Rheumatism. 2006 ; Vol. 54, No. 1. pp. 97-104.
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