Increased expression of connective tissue growth factor in the infarct zone of experimentally induced myocardial infarction in rats

Hiromichi Ohnishi, Takefumi Oka, Shozo Kusachi, Tohru Nakanishi, Kenji Takeda, Makoto Nakahama, Masayuki Doi, Takashi Murakami, Yoshifumi Ninomiya, Masaharu Takigawa, Takao Tsujin

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102 Citations (Scopus)

Abstract

Connective tissue growth factor (CTGF), a 36- to 38-kDa peptide, is selectively induced by transforming growth factor-β and has been suggested to contribute to tissue repair. To test the hypothesis that CTGF is expressed in myocardial infarct tissue following acute myocardial infarction (AMI), we examined CTGF expression after AMI was experimentally induced in rats. Myocardial infarction was induced by left coronary artery ligation in male Sprague-Dawley rats. Northern blotting demonstrated that the CTGF mRNA expression on days 2, 7 and 14 was increased by 6-, 23- and 8-fold, respectively, compared to that in the pre-ligation hearts. In situ hybridization revealed CTGF mRNA signals on day 2 in myocytes in the infarct marginal zone and spindle-shaped mesenchymal cells (presumably myofibroblasts and fibroblasts) located between surviving myocytes in the infarct peripheral zone. On day 7, the signals were observed in the inner lesion of the infarct around infarct granulation tissue. Western blotting demonstrated that the CTGF protein expression on days 2, 7 and 14 was increased compared to the pre-ligation hearts. Immunopositive staining for CTGF was observed in the inner lesion of the infarct tissue on day 7. In conclusion, the findings demonstrated the increased expression of CTGF in the infarct tissue. Myocytes in the infarct marginal zone and spindle-shaped mesenchymal cells (presumably myofibroblasts and fibroblasts) were the cells responsible for CTGF production.

Original languageEnglish
Pages (from-to)2411-2422
Number of pages12
JournalJournal of Molecular and Cellular Cardiology
Volume30
Issue number11
DOIs
Publication statusPublished - Nov 1998

Keywords

  • Electrophoresis
  • Extracellular matrix
  • Immunohistochemistry
  • Ischemic heart disease
  • Molecular biology

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

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  • Cite this

    Ohnishi, H., Oka, T., Kusachi, S., Nakanishi, T., Takeda, K., Nakahama, M., Doi, M., Murakami, T., Ninomiya, Y., Takigawa, M., & Tsujin, T. (1998). Increased expression of connective tissue growth factor in the infarct zone of experimentally induced myocardial infarction in rats. Journal of Molecular and Cellular Cardiology, 30(11), 2411-2422. https://doi.org/10.1006/jmcc.1998.0799