TY - JOUR
T1 - Increased expression of calcium-binding protein S100 in human uterine smooth muscle tumours
AU - Kanamori, Takanobu
AU - Takakura, Kenji
AU - Mandai, Masaki
AU - Kariya, Masatoshi
AU - Fukuhara, Ken
AU - Sakaguchi, Masakiyo
AU - Huh, Nam Ho
AU - Saito, Kaoru
AU - Sakurai, Toshiharu
AU - Fujita, Jun
AU - Fujii, Shingo
N1 - Funding Information:
We are grateful to Ms Michiko Muraoka for her helpful technical support. This work was supported in part by Grants-in-Aid for Scientific Research (No. 13307047 and 13877272) from the Ministry of Education and Sports, Japan.
PY - 2004/10
Y1 - 2004/10
N2 - S100 proteins belong to the EF-hand Ca2+-binding protein family and regulate a variety of cellular processes via interaction with different target proteins. Several diseases, including cancer and melanoma, are related to the abnormal expression of S100 proteins, which are expressed in cell- and tissue-specific manners. We investigated the expression of S100 family members in human uterine smooth muscle tumours. Expression of six members of the S100 protein family: S100A1, A4, A6, A7, A10 and A11, was found in human uterine leiomyoma and myometrium tissue, but expression of other members was not detected by RT-PCR. Real-time PCR showed that S100A11 expression was significantly increased in leiomyoma compared with myometrium. Suppression of S100A11 by small interfering RNA (siRNA) led to apoptosis, and the overexpression of S100A11 inhibited apoptosis in human uterine smooth muscle tumour cells. These findings suggest that S100A11 has an anti-apoptotic function and is related to the process of growth of human uterine leiomyoma.
AB - S100 proteins belong to the EF-hand Ca2+-binding protein family and regulate a variety of cellular processes via interaction with different target proteins. Several diseases, including cancer and melanoma, are related to the abnormal expression of S100 proteins, which are expressed in cell- and tissue-specific manners. We investigated the expression of S100 family members in human uterine smooth muscle tumours. Expression of six members of the S100 protein family: S100A1, A4, A6, A7, A10 and A11, was found in human uterine leiomyoma and myometrium tissue, but expression of other members was not detected by RT-PCR. Real-time PCR showed that S100A11 expression was significantly increased in leiomyoma compared with myometrium. Suppression of S100A11 by small interfering RNA (siRNA) led to apoptosis, and the overexpression of S100A11 inhibited apoptosis in human uterine smooth muscle tumour cells. These findings suggest that S100A11 has an anti-apoptotic function and is related to the process of growth of human uterine leiomyoma.
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U2 - 10.1093/molehr/gah100
DO - 10.1093/molehr/gah100
M3 - Article
C2 - 15322223
AN - SCOPUS:5044252040
SN - 1360-9947
VL - 10
SP - 735
EP - 742
JO - Molecular Human Reproduction
JF - Molecular Human Reproduction
IS - 10
ER -
