Increased expression of biglycan mRNA in pressure-overloaded rat heart

Youko Ayada, Shozo Kusachi, Takashi Murakami, Satoshi Hirohata, Syunji Takemoto, Issei Komatsubara, Junichi Hayashi, Akihiro Iwabu, Yoshifumi Ninomiya, Takao Tsuji

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Abstract

Biglycan mRNA expression in rat myocardium after abdominal aortic banding with renal ischemia was examined. The Northern blot analysis demonstrated that expression of biglycan mRNA in the pressure-overloaded hearts on days 2, 7, 14 and 28 was 2.88±0.89, 2.32±0.49, 2.17±0.57 and 1.81±0.46-fold higher, respectively, than that in the sham-operated hearts. In situ hybridization showed an increased density of biglycan mRNA signal-positive cells in the pressure-overloaded hearts. The cells with positive signals were spindle-shaped mesenchymal cells in the myocardial interstitium. A marked increase in biglycan mRNA signal expression was also observed in endothelial cells and smooth muscle cells of the thickened myocardial capillary wall. These results demonstrated an increase in biglycan mRNA in the pressure-overloaded heart in mesenchymal cells in the myocardial interstitium, and in endothelial and smooth muscle cells of the capillaries, indicating that biglycan contributes to the ventricular and vascular remodeling in response to pressure overload.

Original languageEnglish
Pages (from-to)633-643
Number of pages11
JournalClinical and Experimental Hypertension
Volume23
Issue number8
DOIs
Publication statusPublished - 2001

Fingerprint

Biglycan
Pressure
Messenger RNA
Smooth Muscle Myocytes
Ventricular Remodeling
Northern Blotting
In Situ Hybridization
Myocardium
Ischemia
Endothelial Cells
Kidney

Keywords

  • Extracellular matrix
  • Hypertension
  • Molecular biology
  • Proteoglycan

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Ayada, Y., Kusachi, S., Murakami, T., Hirohata, S., Takemoto, S., Komatsubara, I., ... Tsuji, T. (2001). Increased expression of biglycan mRNA in pressure-overloaded rat heart. Clinical and Experimental Hypertension, 23(8), 633-643. https://doi.org/10.1081/CEH-100107393

Increased expression of biglycan mRNA in pressure-overloaded rat heart. / Ayada, Youko; Kusachi, Shozo; Murakami, Takashi; Hirohata, Satoshi; Takemoto, Syunji; Komatsubara, Issei; Hayashi, Junichi; Iwabu, Akihiro; Ninomiya, Yoshifumi; Tsuji, Takao.

In: Clinical and Experimental Hypertension, Vol. 23, No. 8, 2001, p. 633-643.

Research output: Contribution to journalArticle

Ayada, Y, Kusachi, S, Murakami, T, Hirohata, S, Takemoto, S, Komatsubara, I, Hayashi, J, Iwabu, A, Ninomiya, Y & Tsuji, T 2001, 'Increased expression of biglycan mRNA in pressure-overloaded rat heart', Clinical and Experimental Hypertension, vol. 23, no. 8, pp. 633-643. https://doi.org/10.1081/CEH-100107393
Ayada, Youko ; Kusachi, Shozo ; Murakami, Takashi ; Hirohata, Satoshi ; Takemoto, Syunji ; Komatsubara, Issei ; Hayashi, Junichi ; Iwabu, Akihiro ; Ninomiya, Yoshifumi ; Tsuji, Takao. / Increased expression of biglycan mRNA in pressure-overloaded rat heart. In: Clinical and Experimental Hypertension. 2001 ; Vol. 23, No. 8. pp. 633-643.
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