Increased ER stress during motor neuron degeneration in a transgenic mouse model of amyotrophic lateral sclerosis

Tetsuya Nagata, Hristelina Ilieva, Tetsuro Murakami, Mito Shiote, Hisashi Narai, Yasuyuki Ohta, Takeshi Hayashi, Mikio Shoji, Koji Abe

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The endoplasmic reticulum (ER), which plays important roles in apoptosis, is susceptible to oxidative stress. ER stress is also thought to be involved in the pathogenesis of neurodegenerative diseases. In this study, we investigated whether ER stress is involved in the pathogenesis of amyotrophic lateral sclerosis (ALS) using the anterior part of the lumbar spinal cord of transgenic mice carrying a mutation (G93A) in the superoxide dismutase 1 (SOD1) gene. Western blot and immunohistochemical analyses demonstrated that the expressions of p-PERK and p-elF2α were increased in the microsome fraction (P3) of the lumbar spinal cord at the pre-symptomatic age of 12 weeks (12W), while the expression of activated caspase-12 was increased in the cytoplasmic fraction (S3) of the lumbar spinal cord at both the pre-symptomatic age of 12W and the late symptomatic age of 20W. In contrast, GRP78 did not show any increases in the microsome fraction (P3) of the lumbar spinal cord at either the pre-symptomatic or symptomatic ages. Thus, the present results strongly suggest that the balance between anti- and pro-apoptotic proteins related to ER stress is impaired from the pre-symptomatic stage in this ALS mouse model, and that this imbalance may be related to the pathogenesis of motor neuron degeneration in ALS.

Original languageEnglish
Pages (from-to)767-771
Number of pages5
JournalNeurological Research
Volume29
Issue number8
DOIs
Publication statusPublished - Dec 2007

Keywords

  • ALS
  • Caspase-12
  • Endoplasmic reticulum
  • Oxidative stress
  • PERK
  • SOD1
  • elF2α

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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