Increased concentrations of apo A-I and apo A-II fragments in the serum of patients with hepatocellular carcinoma by magnetic beads-assisted MALDI-TOF mass spectrometry

Yang Liu, Kazuyuki Sogawa, Masahiko Sunaga, Hiroshi Umemura, Mamoru Satoh, Takahiro Kazami, Masaharu Yoshikawa, Takeshi Tomonaga, Osamu Yokosuka, Fumio Nomura

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10 Citations (Scopus)


Objectives: Recent advances in sophisticated technologies in proteomics should provide promising ways to discover novel markers for hepatocellular carcinoma (HCC) in the early diagnosis. Methods: Serum peptide and protein profiling was conducted by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Profiling was carried out in a training set of 16 patients with HCC and a testing set of 15 patients with cirrhosis without HCC. All the patients were hepatitis C virus positive. Candidate peaks were processed to partial purification, followed by protein identification by amino acid sequence analysis. Immunoprecipitation was conducted to confirm the protein identity. Results: Partial purification and protein identification revealed that one peak that was up-regulated in HCC sera both in the training and the testing sets was a fragment of apolipoprotein A-I (apo A-I). Immunoprecipitation confirmed this result. Conclusions: MALDI-TOF MS analysis revealed that apo A-I is a potential novel serum marker of HCC. Combination of these pretreatments and the current magnet bead-assisted MALDI-TOF MS will further enhance the efficiency of biomarker discovery for HCC.

Original languageEnglish
Pages (from-to)52-61
Number of pages10
JournalAmerican Journal of Clinical Pathology
Issue number1
Publication statusPublished - 2014
Externally publishedYes



  • Apolipoprotein A-I
  • Apolipoprotein A-II
  • Hepatocellular carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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