Abstract
Objectives: Recent advances in sophisticated technologies in proteomics should provide promising ways to discover novel markers for hepatocellular carcinoma (HCC) in the early diagnosis. Methods: Serum peptide and protein profiling was conducted by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Profiling was carried out in a training set of 16 patients with HCC and a testing set of 15 patients with cirrhosis without HCC. All the patients were hepatitis C virus positive. Candidate peaks were processed to partial purification, followed by protein identification by amino acid sequence analysis. Immunoprecipitation was conducted to confirm the protein identity. Results: Partial purification and protein identification revealed that one peak that was up-regulated in HCC sera both in the training and the testing sets was a fragment of apolipoprotein A-I (apo A-I). Immunoprecipitation confirmed this result. Conclusions: MALDI-TOF MS analysis revealed that apo A-I is a potential novel serum marker of HCC. Combination of these pretreatments and the current magnet bead-assisted MALDI-TOF MS will further enhance the efficiency of biomarker discovery for HCC.
| Original language | English |
|---|---|
| Pages (from-to) | 52-61 |
| Number of pages | 10 |
| Journal | American Journal of Clinical Pathology |
| Volume | 141 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2014 |
| Externally published | Yes |
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Keywords
- Apolipoprotein A-I
- Apolipoprotein A-II
- Hepatocellular carcinoma
- MALDI-TOF MS
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Increased concentrations of apo A-I and apo A-II fragments in the serum of patients with hepatocellular carcinoma by magnetic beads-assisted MALDI-TOF mass spectrometry. / Liu, Yang; Sogawa, Kazuyuki; Sunaga, Masahiko; Umemura, Hiroshi; Satoh, Mamoru; Kazami, Takahiro; Yoshikawa, Masaharu; Tomonaga, Takeshi; Yokosuka, Osamu; Nomura, Fumio.
In: American Journal of Clinical Pathology, Vol. 141, No. 1, 2014, p. 52-61.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Increased concentrations of apo A-I and apo A-II fragments in the serum of patients with hepatocellular carcinoma by magnetic beads-assisted MALDI-TOF mass spectrometry
AU - Liu, Yang
AU - Sogawa, Kazuyuki
AU - Sunaga, Masahiko
AU - Umemura, Hiroshi
AU - Satoh, Mamoru
AU - Kazami, Takahiro
AU - Yoshikawa, Masaharu
AU - Tomonaga, Takeshi
AU - Yokosuka, Osamu
AU - Nomura, Fumio
PY - 2014
Y1 - 2014
N2 - Objectives: Recent advances in sophisticated technologies in proteomics should provide promising ways to discover novel markers for hepatocellular carcinoma (HCC) in the early diagnosis. Methods: Serum peptide and protein profiling was conducted by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Profiling was carried out in a training set of 16 patients with HCC and a testing set of 15 patients with cirrhosis without HCC. All the patients were hepatitis C virus positive. Candidate peaks were processed to partial purification, followed by protein identification by amino acid sequence analysis. Immunoprecipitation was conducted to confirm the protein identity. Results: Partial purification and protein identification revealed that one peak that was up-regulated in HCC sera both in the training and the testing sets was a fragment of apolipoprotein A-I (apo A-I). Immunoprecipitation confirmed this result. Conclusions: MALDI-TOF MS analysis revealed that apo A-I is a potential novel serum marker of HCC. Combination of these pretreatments and the current magnet bead-assisted MALDI-TOF MS will further enhance the efficiency of biomarker discovery for HCC.
AB - Objectives: Recent advances in sophisticated technologies in proteomics should provide promising ways to discover novel markers for hepatocellular carcinoma (HCC) in the early diagnosis. Methods: Serum peptide and protein profiling was conducted by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Profiling was carried out in a training set of 16 patients with HCC and a testing set of 15 patients with cirrhosis without HCC. All the patients were hepatitis C virus positive. Candidate peaks were processed to partial purification, followed by protein identification by amino acid sequence analysis. Immunoprecipitation was conducted to confirm the protein identity. Results: Partial purification and protein identification revealed that one peak that was up-regulated in HCC sera both in the training and the testing sets was a fragment of apolipoprotein A-I (apo A-I). Immunoprecipitation confirmed this result. Conclusions: MALDI-TOF MS analysis revealed that apo A-I is a potential novel serum marker of HCC. Combination of these pretreatments and the current magnet bead-assisted MALDI-TOF MS will further enhance the efficiency of biomarker discovery for HCC.
KW - Apolipoprotein A-I
KW - Apolipoprotein A-II
KW - Hepatocellular carcinoma
KW - MALDI-TOF MS
UR - http://www.scopus.com/inward/record.url?scp=84894488319&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84894488319&partnerID=8YFLogxK
U2 - 10.1309/AJCPBLFBNAP6N2UN
DO - 10.1309/AJCPBLFBNAP6N2UN
M3 - Article
VL - 141
SP - 52
EP - 61
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
SN - 0002-9173
IS - 1
ER -