Abstract
Objectives: Recent advances in sophisticated technologies in proteomics should provide promising ways to discover novel markers for hepatocellular carcinoma (HCC) in the early diagnosis. Methods: Serum peptide and protein profiling was conducted by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Profiling was carried out in a training set of 16 patients with HCC and a testing set of 15 patients with cirrhosis without HCC. All the patients were hepatitis C virus positive. Candidate peaks were processed to partial purification, followed by protein identification by amino acid sequence analysis. Immunoprecipitation was conducted to confirm the protein identity. Results: Partial purification and protein identification revealed that one peak that was up-regulated in HCC sera both in the training and the testing sets was a fragment of apolipoprotein A-I (apo A-I). Immunoprecipitation confirmed this result. Conclusions: MALDI-TOF MS analysis revealed that apo A-I is a potential novel serum marker of HCC. Combination of these pretreatments and the current magnet bead-assisted MALDI-TOF MS will further enhance the efficiency of biomarker discovery for HCC.
Original language | English |
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Pages (from-to) | 52-61 |
Number of pages | 10 |
Journal | American Journal of Clinical Pathology |
Volume | 141 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 2014 |
Keywords
- Apolipoprotein A-I
- Apolipoprotein A-II
- Hepatocellular carcinoma
- MALDI-TOF MS
ASJC Scopus subject areas
- Pathology and Forensic Medicine