TY - JOUR
T1 - Increase of DC-LAMP+ mature dendritic cell subsets in dermatopathic lymphadenitis of mycosis fungoides
AU - Tada, Kotaro
AU - Hamada, Toshihisa
AU - Asagoe, Kenji
AU - Umemura, Hiroshi
AU - Mizuno-Ikeda, Kazuko
AU - Aoyama, Yumi
AU - Otsuka, Masaki
AU - Yamasaki, Osamu
AU - Iwatsuki, Keiji
N1 - Funding Information:
Disclosure. Financial support: This work was partly supported by a grant from the Ministry of Health, Labour and Welfare (Research for Promotion of Cancer Control Programmes). Conflict of interest: none.
Publisher Copyright:
© 2014, John Libbey Eurotext. All rights reserved.
PY - 2014
Y1 - 2014
N2 - Background: Little is known about the immunological milieu of the skindraining lymph nodes (LNs) in mycosis fungoides (MF). Objectives: We studied dendritic cell (DC) subsets in the dermatopathic lymphadenitis of MF patients. Methods: We immunohistochemically examined DC subsets and their distribution in 16 LN samples from 14 patients with MF (N1 LN, eight patients; N2, four; and N3, four), and we compared them with non-metastatic sentinel LNs from eight patients with melanoma. Results: The number of S-100 protein+ DCs was markedly increased in the LNs from theMFpatients and the major componentwasDC-LAMP+ mature DCs in the outer and paracortex areas, where DC-SIGN+ immature DCs were relatively decreased in proportion. In contrast, DC-SIGN+ cells were relatively increased in proportion compared to DC-LAMP+ cells in the medulla. Although no significant difference was observed in the proportions of CD1a+ or Langerin+ DCs among the N1, N2, and N3 nodes, CD163+ M2-type macrophages were increased in number in the N2 and N3 nodes. Conclusions: Our observations indicate that mature DCs accumulate in the outer and paracortex areas in dermatopathic lymphadenitis and M2-type macrophages might increase in number during disease progression.
AB - Background: Little is known about the immunological milieu of the skindraining lymph nodes (LNs) in mycosis fungoides (MF). Objectives: We studied dendritic cell (DC) subsets in the dermatopathic lymphadenitis of MF patients. Methods: We immunohistochemically examined DC subsets and their distribution in 16 LN samples from 14 patients with MF (N1 LN, eight patients; N2, four; and N3, four), and we compared them with non-metastatic sentinel LNs from eight patients with melanoma. Results: The number of S-100 protein+ DCs was markedly increased in the LNs from theMFpatients and the major componentwasDC-LAMP+ mature DCs in the outer and paracortex areas, where DC-SIGN+ immature DCs were relatively decreased in proportion. In contrast, DC-SIGN+ cells were relatively increased in proportion compared to DC-LAMP+ cells in the medulla. Although no significant difference was observed in the proportions of CD1a+ or Langerin+ DCs among the N1, N2, and N3 nodes, CD163+ M2-type macrophages were increased in number in the N2 and N3 nodes. Conclusions: Our observations indicate that mature DCs accumulate in the outer and paracortex areas in dermatopathic lymphadenitis and M2-type macrophages might increase in number during disease progression.
KW - DC-LAMP
KW - DC-SIGN
KW - Dendritic cell
KW - Dermatopathic lymphadenitis
KW - M2-type macrophage
KW - Mycosis fungoides
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U2 - 10.1684/ejd.2014.2437
DO - 10.1684/ejd.2014.2437
M3 - Article
C2 - 25672788
AN - SCOPUS:84922755803
SN - 1167-1122
VL - 24
SP - 670
EP - 675
JO - European Journal of Dermatology
JF - European Journal of Dermatology
IS - 6
ER -