Increase of ciliary neurotrophic factor (CNTF) in the ischemic rat brain as determined by a sensitive enzyme-linked immunoassay

Jianguo Hu, Tsuyoshi Saito, Koji Abe, Takeo Deguchi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

To study the distribution of ciliary neurotrophic factor (CNTF) in the rat brain under physiological as well as pathological conditions, a sensitive sandwich enzyme linked immunoassay (EIA) for rat CNTF was developed using rabbit anti-CNTF antibody. This method detected as little as 10 pg ml-1 CNTF with a good linearity and accuracy. Analysis of various regions of rat brain and peripheral nerves showed a high content of CNTF in the sciatic nerves, spinal cord, optic nerves, and olfactory bulb. Transient focal ischemia of rat brain caused by middle cerebral artery occlusion significantly and specifically increased CNTF levels in the cerebral cortex and hippocampus regions in the ischemic hemisphere.

Original languageEnglish
Pages (from-to)593-598
Number of pages6
JournalNeurological Research
Volume19
Issue number6
DOIs
Publication statusPublished - Jan 1 1997
Externally publishedYes

Keywords

  • CNTF
  • EIA
  • Ischemic brain
  • Rat brain
  • Recombinant rat CNTF

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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