Increase in Fe2+-producing activity during growth of Acidithiobacillus ferrooxidans ATCC23270 on sulfur

Tsuyoshi Sugio, Taher M. Taha, Tadayoshi Kanao, Fumiaki Takeuchi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

When Acidithiobacillus ferrooxidans ATCC23270 cells, grown for many generations on sulfur were grown in sulfur medium with and without Fe 2+, the bacterium markedly increased not only in iron oxidase activity but also in Fe2+-producing sulfide:ferric ion oxidoreductase (SFORase) activity during the early log phase, and retained part of these activities during the late log phase. The activity of SFORase, which catalyzes the production of Fe2+ from Fe2+ and sulfur, of sulfur-grown cells was approximately 10-20 fold higher than that of iron-grown cells. aa3 type cytochrome c oxidase, an important component of iron oxidase in A. ferrooxidans, was partially purified from sulfur-grown cells. A. ferrooxidans ATCC23270 cells grown for many generations on sulfur had the ability to grow on iron as rapidly as that did iron-grown cells. These results suggest that both iron oxidase and Fe2+-producing SFORase have a role in the energy generation of A. ferrooxidans ATCC23270 from sulfur.

Original languageEnglish
Pages (from-to)2663-2669
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume71
Issue number11
DOIs
Publication statusPublished - Nov 30 2007

Keywords

  • Acidithiobacillus ferrooxidans
  • Cytochrome c oxidase
  • Ferrous iron production
  • Iron oxidase
  • Sulfur oxidation

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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