Increase in cell adhesiveness on a poly(ethylene terephthalate) fabric by sintered hydroxyapatite nanocrystal coating in the development of an artificial blood vessel

Tsutomu Furuzono, Miwa Masuda, Masahiro Okada, Shoji Yasuda, Hiroyuki Kadono, Ryoichi Tanaka, Kunio Miyatake

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Nano-scaled sintered hydroxyapatite (HAp) crystals were covalently linked onto a poly(ethylene terephthalate) (PET) fabric substrate chemically modified by graft polymerization with γ-methacryloxypropyl triethoxysilane (MPTS) for development of an artificial blood vessel. The weight gain of graft polymerization with poly(MPTS) on PET in benzyl alcohol containing H2O2 as an initiator increased as increasing the reaction time and finally reached a plateau value of about 3.5 wt%. The surface characterization of surface modification with poly(MPTS)-grafting was conducted by x-ray photoelectron spectroscopy. HAp nanocrystals of approximately 50 nm in diameter, monodispersed in pure ethanol, were coupled with alkoxysilyl groups of the poly(MPTS)-grafted PET substrate. The HAp nanocrystals were uniformly and strongly coated on the surface of the PET fabrics, although HAp particles adsorbed physically on the original PET without poly(MPTS) grafting were almost removed by ultrasonic wave treatment. More human umbilical vein endothelial cells adhered to the HAp/PET composite fabric compared with original PET after only 4 hours of initial incubation, and the same was observed on the collagen-coated PET. The coating of sintered HAp nanocrystals imparted bioactivity to the polyester substrate, which is a widely used biomedical polymer, without a coating of adhesion proteins derived from animals, such as collagen or gelatin. A prototype of an artificial blood vessel was finally fabricated by use of HAp/PET composite.

Original languageEnglish
Pages (from-to)315-320
Number of pages6
JournalASAIO Journal
Volume52
Issue number3
DOIs
Publication statusPublished - May 2006
Externally publishedYes

Fingerprint

Blood vessel prostheses
Blood Substitutes
Adhesiveness
Polyethylene Terephthalates
Durapatite
Hydroxyapatite
Polyethylene terephthalates
Nanoparticles
Nanocrystals
Blood Vessels
Coatings
Collagen
Grafts
Polymerization
Substrates
Benzyl Alcohol
Transplants
Photoelectron Spectroscopy
Polyesters
Ultrasonic waves

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering

Cite this

Increase in cell adhesiveness on a poly(ethylene terephthalate) fabric by sintered hydroxyapatite nanocrystal coating in the development of an artificial blood vessel. / Furuzono, Tsutomu; Masuda, Miwa; Okada, Masahiro; Yasuda, Shoji; Kadono, Hiroyuki; Tanaka, Ryoichi; Miyatake, Kunio.

In: ASAIO Journal, Vol. 52, No. 3, 05.2006, p. 315-320.

Research output: Contribution to journalArticle

Furuzono, Tsutomu ; Masuda, Miwa ; Okada, Masahiro ; Yasuda, Shoji ; Kadono, Hiroyuki ; Tanaka, Ryoichi ; Miyatake, Kunio. / Increase in cell adhesiveness on a poly(ethylene terephthalate) fabric by sintered hydroxyapatite nanocrystal coating in the development of an artificial blood vessel. In: ASAIO Journal. 2006 ; Vol. 52, No. 3. pp. 315-320.
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