Increase in CD95 (Fas/APO-1)-positive CD4+ and CD8+ T cells in peripheral blood derived from patients with autoimmune hepatitis or chronic hepatitis C with autoimmune phenomena

Shin Ogawa, Kohsaku Sakaguchi, Akinobu Takaki, Kunihiro Shiraga, Tomoyuki Sawayama, Hirokazu Mouri, Manabi Miyashita, Norio Koide, Takao Tsuji

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: The expressions of CD95 (Fas/APO-1) and Bcl-2 are determinants of apoptosis in normal lymphocytes, and abnormalities in their expressions might contribute to the induction of autoimmunity. In this study, we examined the expressions of CD95 and Bcl-2 on freshly isolated T and B cells from patients with autoimmune hepatitis (AIH) or chronic hepatitis C associated with autoimmune phenomena (CH-C(AI)). Methods: The CD95 and Bcl-2 expressions within CD4+ T, CD8+ T, and CD19+ B cell subsets were analysed by two-colour flow cytometry. Results: The surface expression of CD95 was significantly high in both the CD4+ T and CD8+ T cell subsets derived from the patients with AIH and those with CH-C(AI), compared with expression in patients with CH-C and normal subjects. The increase in CD95 expression was associated with the phenotypic conversion of naive CD45RO- to primed CD45RO+ CD4+T cells. Bcl-2 was detected in the vast majority of peripheral T and B cells. There was no significant difference in the percentage of Bcl2- positive cells in the CD4+T cell, CD8+T cell and CD19+ B cell subsets among the patient groups and normal subjects. Conclusions: These results indicate that an increase in CD4+T cells expressing CD45RO and CD95 marks an important subset of AIH and CH-C(AI) patients. These expanded CD95+ CD45RO+ primed T cells most likely reflect a continuous antigen-specific or non- specific activation oft lymphocytes, and/or the persistent presence of activated lymphocytes as a consequence of abnormalities in the peripheral deletion of activated lymphocytes. These persistently activated lymphocytes might play a role in the induction of autoimmunity in AIH and CH-C(AI). (C) 2000 Blackwell Science Asia Pty Ltd.

Original languageEnglish
Pages (from-to)69-75
Number of pages7
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume15
Issue number1
DOIs
Publication statusPublished - Jan 1 2000

Keywords

  • Autoimmune hepatitis
  • Autoimmunity
  • Bcl-2
  • CD45 RO
  • CD95
  • Chronic hepatitis C

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Fingerprint Dive into the research topics of 'Increase in CD95 (Fas/APO-1)-positive CD4<sup>+</sup> and CD8<sup>+</sup> T cells in peripheral blood derived from patients with autoimmune hepatitis or chronic hepatitis C with autoimmune phenomena'. Together they form a unique fingerprint.

  • Cite this