Increase in beating rate of cultured chick cardiac myocytes by ethanol and inhibition of the increase by antiarrhythmic drugs

Kazufumi Nakamura, Hirosuke Kouchi, Tohru Ohe, Masayoshi Namba

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Drinking alcohol sometimes causes cardiac arrhythmia, but the precise mechanism remains unknown. To study the mechanism, we investigated the effects of ethanol exposure on the beating rate of cultured chick cardiac myocytes. Primary cultures of cardiac myocytes were prepared from the ventricles of 14-day-old chick embryos and then treated with ethanol which, in the range of 0.3 to 1.5 vol%, increased the beating rate in a dose- dependent manner. Ethanol (0.6 vol%) caused an increase in the beating rate, but disopyramide (5 μg/ml) and procainamide (10 μg/ml), Na+ and K+ channel blockers, inhibited the increase in the beating rate significantly. Neither lidocaine (5 μg/ml) nor mexiletine (2 μg/ml), Na+ channel blockers, nor calcium antagonist verapamil (5 ng/ml) inhibited the increase. However, tetraethylammonium chloride (ranging from 15 to 30 mmol/l), a K+ channel blocker, inhibited the increase. These findings indicate that ethanol increases the beating rate of cultured chick cardiac myocytes via the activation of the K+ channel. This experimental model may be useful in studying the effect of ethanol on the K+ channel.

Original languageEnglish
JournalAlcoholism: Clinical and Experimental Research
Volume23
Issue number4 SUPPL.
Publication statusPublished - Apr 1999

Fingerprint

Anti-Arrhythmia Agents
Cardiac Myocytes
Ethanol
Mexiletine
Disopyramide
Procainamide
Tetraethylammonium
Calcium Channel Blockers
Chick Embryo
Verapamil
Lidocaine
Alcohol Drinking
Cardiac Arrhythmias
Theoretical Models
Chemical activation
Alcohols
Inhibition (Psychology)
Calcium

Keywords

  • Arrhythmia
  • Cardiac Myocytes
  • Ethanol
  • Potassium Channel

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Increase in beating rate of cultured chick cardiac myocytes by ethanol and inhibition of the increase by antiarrhythmic drugs. / Nakamura, Kazufumi; Kouchi, Hirosuke; Ohe, Tohru; Namba, Masayoshi.

In: Alcoholism: Clinical and Experimental Research, Vol. 23, No. 4 SUPPL., 04.1999.

Research output: Contribution to journalArticle

@article{c0ea87266d16477ea08e00ed1db56611,
title = "Increase in beating rate of cultured chick cardiac myocytes by ethanol and inhibition of the increase by antiarrhythmic drugs",
abstract = "Drinking alcohol sometimes causes cardiac arrhythmia, but the precise mechanism remains unknown. To study the mechanism, we investigated the effects of ethanol exposure on the beating rate of cultured chick cardiac myocytes. Primary cultures of cardiac myocytes were prepared from the ventricles of 14-day-old chick embryos and then treated with ethanol which, in the range of 0.3 to 1.5 vol{\%}, increased the beating rate in a dose- dependent manner. Ethanol (0.6 vol{\%}) caused an increase in the beating rate, but disopyramide (5 μg/ml) and procainamide (10 μg/ml), Na+ and K+ channel blockers, inhibited the increase in the beating rate significantly. Neither lidocaine (5 μg/ml) nor mexiletine (2 μg/ml), Na+ channel blockers, nor calcium antagonist verapamil (5 ng/ml) inhibited the increase. However, tetraethylammonium chloride (ranging from 15 to 30 mmol/l), a K+ channel blocker, inhibited the increase. These findings indicate that ethanol increases the beating rate of cultured chick cardiac myocytes via the activation of the K+ channel. This experimental model may be useful in studying the effect of ethanol on the K+ channel.",
keywords = "Arrhythmia, Cardiac Myocytes, Ethanol, Potassium Channel",
author = "Kazufumi Nakamura and Hirosuke Kouchi and Tohru Ohe and Masayoshi Namba",
year = "1999",
month = "4",
language = "English",
volume = "23",
journal = "Alcoholism: Clinical and Experimental Research",
issn = "0145-6008",
publisher = "Wiley-Blackwell",
number = "4 SUPPL.",

}

TY - JOUR

T1 - Increase in beating rate of cultured chick cardiac myocytes by ethanol and inhibition of the increase by antiarrhythmic drugs

AU - Nakamura, Kazufumi

AU - Kouchi, Hirosuke

AU - Ohe, Tohru

AU - Namba, Masayoshi

PY - 1999/4

Y1 - 1999/4

N2 - Drinking alcohol sometimes causes cardiac arrhythmia, but the precise mechanism remains unknown. To study the mechanism, we investigated the effects of ethanol exposure on the beating rate of cultured chick cardiac myocytes. Primary cultures of cardiac myocytes were prepared from the ventricles of 14-day-old chick embryos and then treated with ethanol which, in the range of 0.3 to 1.5 vol%, increased the beating rate in a dose- dependent manner. Ethanol (0.6 vol%) caused an increase in the beating rate, but disopyramide (5 μg/ml) and procainamide (10 μg/ml), Na+ and K+ channel blockers, inhibited the increase in the beating rate significantly. Neither lidocaine (5 μg/ml) nor mexiletine (2 μg/ml), Na+ channel blockers, nor calcium antagonist verapamil (5 ng/ml) inhibited the increase. However, tetraethylammonium chloride (ranging from 15 to 30 mmol/l), a K+ channel blocker, inhibited the increase. These findings indicate that ethanol increases the beating rate of cultured chick cardiac myocytes via the activation of the K+ channel. This experimental model may be useful in studying the effect of ethanol on the K+ channel.

AB - Drinking alcohol sometimes causes cardiac arrhythmia, but the precise mechanism remains unknown. To study the mechanism, we investigated the effects of ethanol exposure on the beating rate of cultured chick cardiac myocytes. Primary cultures of cardiac myocytes were prepared from the ventricles of 14-day-old chick embryos and then treated with ethanol which, in the range of 0.3 to 1.5 vol%, increased the beating rate in a dose- dependent manner. Ethanol (0.6 vol%) caused an increase in the beating rate, but disopyramide (5 μg/ml) and procainamide (10 μg/ml), Na+ and K+ channel blockers, inhibited the increase in the beating rate significantly. Neither lidocaine (5 μg/ml) nor mexiletine (2 μg/ml), Na+ channel blockers, nor calcium antagonist verapamil (5 ng/ml) inhibited the increase. However, tetraethylammonium chloride (ranging from 15 to 30 mmol/l), a K+ channel blocker, inhibited the increase. These findings indicate that ethanol increases the beating rate of cultured chick cardiac myocytes via the activation of the K+ channel. This experimental model may be useful in studying the effect of ethanol on the K+ channel.

KW - Arrhythmia

KW - Cardiac Myocytes

KW - Ethanol

KW - Potassium Channel

UR - http://www.scopus.com/inward/record.url?scp=0032908298&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032908298&partnerID=8YFLogxK

M3 - Article

C2 - 10235285

AN - SCOPUS:0032908298

VL - 23

JO - Alcoholism: Clinical and Experimental Research

JF - Alcoholism: Clinical and Experimental Research

SN - 0145-6008

IS - 4 SUPPL.

ER -