Increase in antibiotic resistant Helicobacter pylori in a University Hospital in Japan

Chihiro Kageyama, Mayu Sato, Hiroyuki Sakae, Yuka Obayashi, Yoshiro Kawahara, Takehiko Mima, Osamu Matsushita, Kenji Yokota, Motowo Mizuno, Hiroyuki Okada

Research output: Contribution to journalArticle

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Abstract

Background: Eradication effectively prevents Helicobacter pylori-associated diseases; however, H. pylori antibiotic resistance has increased throughout Japan and worldwide. This study aimed to assess rates of resistance to antibiotics; amoxicillin, clarithromycin and metronidazole in a University Hospital in Japan. Materials and methods: H. pylori (208 strains) were isolated from patients at the Okayama University Hospital in Japan. The minimum inhibitory concentrations (MIC) were determined using the mean values of the E-test to determine the antimicrobial susceptibilities of the strains. Sequencing and gene analysis were performed to analyze resistance genes to clarithromycin and amoxicillin. Results: Rates of amoxicillin, clarithromycin, and metronidazole resistance were 13%, 48%, and 49%, respectively. Genetic analysis indicated that the A2143G point mutation in 23S rDNA is closely associated with the MIC of clarithromycin. The MIC in amoxicillin-resistant strains increased with an increase in the number of PBP1A amino acids mutations. Conclusion: Genetic analysis for resistant strains is not clinically effective in cases of amoxicillin resistance. Numerous bacteria with already high antibiotic resistance rates have been isolated in large hospitals such as a University Hospital. For effective eradication therapy, MIC measurement should be considered via several methods.

Original languageEnglish
Pages (from-to)597-602
Number of pages6
JournalInfection and Drug Resistance
Volume12
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Amoxicillin
Helicobacter pylori
Clarithromycin
Microbial Sensitivity Tests
Japan
Anti-Bacterial Agents
Microbial Drug Resistance
Metronidazole
Ribosomal DNA
Point Mutation
Genes
Bacteria
Amino Acids
Mutation

Keywords

  • Amoxicillin
  • Clarithromycin
  • Genotype
  • Helicobacter pylori
  • Resistance
  • University hospital

ASJC Scopus subject areas

  • Pharmacology
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

Increase in antibiotic resistant Helicobacter pylori in a University Hospital in Japan. / Kageyama, Chihiro; Sato, Mayu; Sakae, Hiroyuki; Obayashi, Yuka; Kawahara, Yoshiro; Mima, Takehiko; Matsushita, Osamu; Yokota, Kenji; Mizuno, Motowo; Okada, Hiroyuki.

In: Infection and Drug Resistance, Vol. 12, 01.01.2019, p. 597-602.

Research output: Contribution to journalArticle

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