Inactivation of Vibrio vulnificus hemolysin through mutation of the N- or C-terminus of the lectin-like domain

Shin-ichi Miyoshi, Yuki Abe, Mitsutoshi Senoh, Tamaki Mizuno, Yoko Maehara, Hiroshi Nakao

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Vibrio vulnificus is an etiological agent causing serious systemic infections in the immunocompromised humans or cultured eels. This species commonly produces a hemolytic toxin consisting of the cytolysin domain and the lectin-like domain. For hemolysis, the lectin-like domain specifically binds to cholesterol in the erythrocyte membrane, and to form a hollow oligomer, the toxin is subsequently assembled on the membrane. The cytolysin domain is essential for the process to form the oligomer. Three-dimensional structure model revealed that two domains connected linearly and the C-terminus was located near to the joint of the domains. Insertion of amino acid residues between two domains was found to cause inactivation of the toxin. In the C-terminus, deletion, substitution or addition of an amino acid residue also elicited reduction of the activity. However, the cholesterol-binding ability was not affected by the mutations. These results suggest that mutation of the C- or N-terminus of the lectin-like domain may result in blockage of the toxin assembly.

Original languageEnglish
Pages (from-to)904-908
Number of pages5
JournalToxicon
Volume57
Issue number6
DOIs
Publication statusPublished - May 2011

Fingerprint

Vibrio vulnificus
Hemolysin Proteins
Lectins
Perforin
Oligomers
Mutation
Cholesterol
Membranes
Amino Acids
Eels
Erythrocyte Membrane
Model structures
Hemolysis
Substitution reactions
Joints
Infection
Vibrio hemolysin

Keywords

  • Cell-free translation
  • Hemolysin
  • Site-directed mutagenesis
  • Vibrio vulnificus

ASJC Scopus subject areas

  • Toxicology

Cite this

Inactivation of Vibrio vulnificus hemolysin through mutation of the N- or C-terminus of the lectin-like domain. / Miyoshi, Shin-ichi; Abe, Yuki; Senoh, Mitsutoshi; Mizuno, Tamaki; Maehara, Yoko; Nakao, Hiroshi.

In: Toxicon, Vol. 57, No. 6, 05.2011, p. 904-908.

Research output: Contribution to journalArticle

Miyoshi, Shin-ichi ; Abe, Yuki ; Senoh, Mitsutoshi ; Mizuno, Tamaki ; Maehara, Yoko ; Nakao, Hiroshi. / Inactivation of Vibrio vulnificus hemolysin through mutation of the N- or C-terminus of the lectin-like domain. In: Toxicon. 2011 ; Vol. 57, No. 6. pp. 904-908.
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