Inactivation of the mitogenic property of pokeweed mitogen by erythrocytes

Ryuzo Ohno, Yoshihisa Kodera, Kohji Ezaki, Mitsune Tanimoto, Kohji Iwamoto, Kazumasa Yamada

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In whole blood culture, pokeweed mitogen (PWM) is unable to stimulate human lymphocytes. This is because the mitogenic property of PWM is inactivated by erythrocytes, presumably due to absorption. The inactivation was observed with as few as 5 × 106/ml of human erythrocytes. Therefore, when PWM is used to study the functions of human lymphocytes, especially of suppressor T lymphocytes, erythrocytes should be removed from lymphocyte preparations for accurate analysis.

Original languageEnglish
Pages (from-to)65-69
Number of pages5
JournalJournal of Immunological Methods
Volume31
Issue number1-2
DOIs
Publication statusPublished - Dec 14 1979

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Ohno, R., Kodera, Y., Ezaki, K., Tanimoto, M., Iwamoto, K., & Yamada, K. (1979). Inactivation of the mitogenic property of pokeweed mitogen by erythrocytes. Journal of Immunological Methods, 31(1-2), 65-69. https://doi.org/10.1016/0022-1759(79)90286-2