Inactivation of photosystems I and II in response to osmotic stress in Synechococcus. Contribution of water channels

Suleyman Allakhverdiev, Atsushi Sakamoto, Yoshitaka Nishiyama, Norio Murata

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

The effects of osmotic stress due to sorbitol on the photosynthetic machinery were investigated in the cyanobacterium Synechococcus R-2. Incubation of cells in 1.0 M sorbitol inactivated photosystems I and II and decreased the intracellular solute space by 50%. These effects of sorbitol were reversible: Photosynthetic activity and cytoplasmic volume returned to the original values after removal of the osmotic stress. A blocker of water channels prevented the osmotic-stress-induced inactivation and shrinkage of the intracellular space. It also prevented the recovery of photosynthetic activity and cytoplasmic volume when applied just before release from osmotic stress. Inhibition of protein synthesis by lincomycin had no significant effects on the inactivation and recovery processes, an observation that suggests that protein synthesis was not involved in these processes. Our results suggest that osmotic stress decreased the amount of water in the cytoplasm via the efflux of water through water channels (aquaporins), with resultant increases in intracellular concentrations of ions and a decrease in photosynthetic activity.

Original languageEnglish
Pages (from-to)1201-1208
Number of pages8
JournalPlant Physiology
Volume122
Issue number4
DOIs
Publication statusPublished - Jan 1 2000
Externally publishedYes

Fingerprint

Synechococcus
Photosystem I Protein Complex
Osmoregulation
Aquaporins
Photosystem II Protein Complex
photosystem I
Osmotic Pressure
osmotic stress
photosystem II
inactivation
Sorbitol
sorbitol
Intracellular Space
water
protein synthesis
Lincomycin
lincomycin
aquaporins
Water
Cyanobacteria

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Inactivation of photosystems I and II in response to osmotic stress in Synechococcus. Contribution of water channels. / Allakhverdiev, Suleyman; Sakamoto, Atsushi; Nishiyama, Yoshitaka; Murata, Norio.

In: Plant Physiology, Vol. 122, No. 4, 01.01.2000, p. 1201-1208.

Research output: Contribution to journalArticle

Allakhverdiev, Suleyman ; Sakamoto, Atsushi ; Nishiyama, Yoshitaka ; Murata, Norio. / Inactivation of photosystems I and II in response to osmotic stress in Synechococcus. Contribution of water channels. In: Plant Physiology. 2000 ; Vol. 122, No. 4. pp. 1201-1208.
@article{de85fa1886f44975bd588e6f9f83fc13,
title = "Inactivation of photosystems I and II in response to osmotic stress in Synechococcus. Contribution of water channels",
abstract = "The effects of osmotic stress due to sorbitol on the photosynthetic machinery were investigated in the cyanobacterium Synechococcus R-2. Incubation of cells in 1.0 M sorbitol inactivated photosystems I and II and decreased the intracellular solute space by 50{\%}. These effects of sorbitol were reversible: Photosynthetic activity and cytoplasmic volume returned to the original values after removal of the osmotic stress. A blocker of water channels prevented the osmotic-stress-induced inactivation and shrinkage of the intracellular space. It also prevented the recovery of photosynthetic activity and cytoplasmic volume when applied just before release from osmotic stress. Inhibition of protein synthesis by lincomycin had no significant effects on the inactivation and recovery processes, an observation that suggests that protein synthesis was not involved in these processes. Our results suggest that osmotic stress decreased the amount of water in the cytoplasm via the efflux of water through water channels (aquaporins), with resultant increases in intracellular concentrations of ions and a decrease in photosynthetic activity.",
author = "Suleyman Allakhverdiev and Atsushi Sakamoto and Yoshitaka Nishiyama and Norio Murata",
year = "2000",
month = "1",
day = "1",
doi = "10.1104/pp.122.4.1201",
language = "English",
volume = "122",
pages = "1201--1208",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "4",

}

TY - JOUR

T1 - Inactivation of photosystems I and II in response to osmotic stress in Synechococcus. Contribution of water channels

AU - Allakhverdiev, Suleyman

AU - Sakamoto, Atsushi

AU - Nishiyama, Yoshitaka

AU - Murata, Norio

PY - 2000/1/1

Y1 - 2000/1/1

N2 - The effects of osmotic stress due to sorbitol on the photosynthetic machinery were investigated in the cyanobacterium Synechococcus R-2. Incubation of cells in 1.0 M sorbitol inactivated photosystems I and II and decreased the intracellular solute space by 50%. These effects of sorbitol were reversible: Photosynthetic activity and cytoplasmic volume returned to the original values after removal of the osmotic stress. A blocker of water channels prevented the osmotic-stress-induced inactivation and shrinkage of the intracellular space. It also prevented the recovery of photosynthetic activity and cytoplasmic volume when applied just before release from osmotic stress. Inhibition of protein synthesis by lincomycin had no significant effects on the inactivation and recovery processes, an observation that suggests that protein synthesis was not involved in these processes. Our results suggest that osmotic stress decreased the amount of water in the cytoplasm via the efflux of water through water channels (aquaporins), with resultant increases in intracellular concentrations of ions and a decrease in photosynthetic activity.

AB - The effects of osmotic stress due to sorbitol on the photosynthetic machinery were investigated in the cyanobacterium Synechococcus R-2. Incubation of cells in 1.0 M sorbitol inactivated photosystems I and II and decreased the intracellular solute space by 50%. These effects of sorbitol were reversible: Photosynthetic activity and cytoplasmic volume returned to the original values after removal of the osmotic stress. A blocker of water channels prevented the osmotic-stress-induced inactivation and shrinkage of the intracellular space. It also prevented the recovery of photosynthetic activity and cytoplasmic volume when applied just before release from osmotic stress. Inhibition of protein synthesis by lincomycin had no significant effects on the inactivation and recovery processes, an observation that suggests that protein synthesis was not involved in these processes. Our results suggest that osmotic stress decreased the amount of water in the cytoplasm via the efflux of water through water channels (aquaporins), with resultant increases in intracellular concentrations of ions and a decrease in photosynthetic activity.

UR - http://www.scopus.com/inward/record.url?scp=0033996023&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033996023&partnerID=8YFLogxK

U2 - 10.1104/pp.122.4.1201

DO - 10.1104/pp.122.4.1201

M3 - Article

C2 - 10759516

AN - SCOPUS:0033996023

VL - 122

SP - 1201

EP - 1208

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 4

ER -