Abstract
We examined the effects of o-phenanthroline and LiClO4 on oxygen evolution and electron transport in the Photosystem 2 complex of the pea. Treatment of Photosystem 2 particles with a combination of 3.0 mM o-phenanthroline and 1.0 M LiClO4 for 30-40 min at 0°C decreased the oxygen-evolving activity with the electron acceptor (either phenyl-p-benzoquinone or 2,6-dichlorophenol indophenol) to less than 5% of the original level. However with the same treatment, the electron-transport activity from an artificial electron donor, 1,5-diphenylcarbohydrazide, to 2,6-dichlorophenol indophenol remained at 60% of the original activity. The amount of manganese in the Photosystem 2 complex decreased in parallel with the loss of oxygen evolution following treatment. These observations suggest that the treatment of the Photosystem 2 complex with o-phenanthroline and LiClO4 inhibits electron transport on the oxygen-evolving side much more significantly than on the electron-acceptor side.
Original language | English |
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Pages (from-to) | 345-349 |
Number of pages | 5 |
Journal | Photosynthesis research |
Volume | 35 |
Issue number | 3 |
DOIs | |
Publication status | Published - Mar 1 1993 |
Keywords
- Key words
- LiClO
- Mn cluster
- Photosystem 2
- electron transport
- o-phenanthroline
- oxygen evolution
ASJC Scopus subject areas
- Biochemistry
- Plant Science
- Cell Biology