Inactivation of photosynthetic oxygen evolution by o-phenanthroline and LiClO4 in Photosystem 2 of the pea

Suleyman I. Allakhverdiev, Hidenori Hayashi, Yoko Fujimura, Vyacheslav V. Klimov, Norio Murata

Research output: Contribution to journalArticle

Abstract

We examined the effects of o-phenanthroline and LiClO4 on oxygen evolution and electron transport in the Photosystem 2 complex of the pea. Treatment of Photosystem 2 particles with a combination of 3.0 mM o-phenanthroline and 1.0 M LiClO4 for 30-40 min at 0°C decreased the oxygen-evolving activity with the electron acceptor (either phenyl-p-benzoquinone or 2,6-dichlorophenol indophenol) to less than 5% of the original level. However with the same treatment, the electron-transport activity from an artificial electron donor, 1,5-diphenylcarbohydrazide, to 2,6-dichlorophenol indophenol remained at 60% of the original activity. The amount of manganese in the Photosystem 2 complex decreased in parallel with the loss of oxygen evolution following treatment. These observations suggest that the treatment of the Photosystem 2 complex with o-phenanthroline and LiClO4 inhibits electron transport on the oxygen-evolving side much more significantly than on the electron-acceptor side.

Original languageEnglish
Pages (from-to)345-349
Number of pages5
JournalPhotosynthesis research
Volume35
Issue number3
DOIs
Publication statusPublished - Mar 1 1993

Keywords

  • Key words
  • LiClO
  • Mn cluster
  • Photosystem 2
  • electron transport
  • o-phenanthroline
  • oxygen evolution

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'Inactivation of photosynthetic oxygen evolution by o-phenanthroline and LiClO<sub>4</sub> in Photosystem 2 of the pea'. Together they form a unique fingerprint.

  • Cite this