Inactivation of photosynthetic oxygen evolution by o-phenanthroline and LiClO4 in Photosystem 2 of the pea

Suleyman I. Allakhverdiev, Hidenori Hayashi, Yoko Fujimura, Vyacheslav V. Klimov, Norio Murata

    Research output: Contribution to journalArticlepeer-review

    Abstract

    We examined the effects of o-phenanthroline and LiClO4 on oxygen evolution and electron transport in the Photosystem 2 complex of the pea. Treatment of Photosystem 2 particles with a combination of 3.0 mM o-phenanthroline and 1.0 M LiClO4 for 30-40 min at 0°C decreased the oxygen-evolving activity with the electron acceptor (either phenyl-p-benzoquinone or 2,6-dichlorophenol indophenol) to less than 5% of the original level. However with the same treatment, the electron-transport activity from an artificial electron donor, 1,5-diphenylcarbohydrazide, to 2,6-dichlorophenol indophenol remained at 60% of the original activity. The amount of manganese in the Photosystem 2 complex decreased in parallel with the loss of oxygen evolution following treatment. These observations suggest that the treatment of the Photosystem 2 complex with o-phenanthroline and LiClO4 inhibits electron transport on the oxygen-evolving side much more significantly than on the electron-acceptor side.

    Original languageEnglish
    Pages (from-to)345-349
    Number of pages5
    JournalPhotosynthesis research
    Volume35
    Issue number3
    DOIs
    Publication statusPublished - Mar 1 1993

    Keywords

    • Key words
    • LiClO
    • Mn cluster
    • Photosystem 2
    • electron transport
    • o-phenanthroline
    • oxygen evolution

    ASJC Scopus subject areas

    • Biochemistry
    • Plant Science
    • Cell Biology

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