In vivo visualization of heterogeneous intratumoral distribution of hypoxia-inducible factor-1α activity by the fusion of high-resolution SPECT and morphological imaging tests

Hirofumi Fujii, Masayuki Yamaguchi, Kazumasa Inoue, Yasuko Mutou, Masashi Ueda, Hideo Saji, Shinae Kizaka-Kondoh, Noriyuki Moriyama, Izumi O. Umeda

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Purpose. We aimed to clearly visualize heterogeneous distribution of hypoxia-inducible factor 1 (HIF) activity in tumor tissues in vivo. Methods. We synthesized of 125I-IPOS, a 125I labeled chimeric protein probe, that would visualize HIF activity. The biodistribution of 125I-IPOS in FM3A tumor-bearing mice was evaluated. Then, the intratumoral localization of this probe was observed by autoradiography, and it was compared with histopathological findings. The distribution of 125I-IPOS in tumors was imaged by a small animal SPECT/CT scanner. The obtained in vivo SPECT-CT fusion images were compared with ex vivo images of excised tumors. Fusion imaging with MRI was also examined. Results. 125I-IPOS well accumulated in FM3A tumors. The intratumoral distribution of 125I-IPOS by autoradiography was quite heterogeneous, and it partially overlapped with that of pimonidazole. High-resolution SPECT-CT fusion images successfully demonstrated the heterogeneity of 125I-IPOS distribution inside tumors. SPECT-MRI fusion images could give more detailed information about the intratumoral distribution of 125I-IPOS. Conclusion. High-resolution SPECT images successfully demonstrated heterogeneous intratumoral distribution of 125I-IPOS. SPECT-CT fusion images, more favorably SPECT-MRI fusion images, would be useful to understand the features of heterogeneous intratumoral expression of HIF activity in vivo.

Original languageEnglish
Article number262741
JournalJournal of Biomedicine and Biotechnology
Volume2012
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Hypoxia-Inducible Factor 1
Single-Photon Emission-Computed Tomography
Image fusion
Tumors
Visualization
Imaging techniques
Magnetic resonance imaging
Neoplasms
Autoradiography
Bearings (structural)
Image resolution
Animals
Tissue
Single Photon Emission Computed Tomography Computed Tomography
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Genetics
  • Molecular Biology
  • Health, Toxicology and Mutagenesis
  • Medicine(all)

Cite this

In vivo visualization of heterogeneous intratumoral distribution of hypoxia-inducible factor-1α activity by the fusion of high-resolution SPECT and morphological imaging tests. / Fujii, Hirofumi; Yamaguchi, Masayuki; Inoue, Kazumasa; Mutou, Yasuko; Ueda, Masashi; Saji, Hideo; Kizaka-Kondoh, Shinae; Moriyama, Noriyuki; Umeda, Izumi O.

In: Journal of Biomedicine and Biotechnology, Vol. 2012, 262741, 2012.

Research output: Contribution to journalArticle

Fujii, Hirofumi ; Yamaguchi, Masayuki ; Inoue, Kazumasa ; Mutou, Yasuko ; Ueda, Masashi ; Saji, Hideo ; Kizaka-Kondoh, Shinae ; Moriyama, Noriyuki ; Umeda, Izumi O. / In vivo visualization of heterogeneous intratumoral distribution of hypoxia-inducible factor-1α activity by the fusion of high-resolution SPECT and morphological imaging tests. In: Journal of Biomedicine and Biotechnology. 2012 ; Vol. 2012.
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abstract = "Purpose. We aimed to clearly visualize heterogeneous distribution of hypoxia-inducible factor 1 (HIF) activity in tumor tissues in vivo. Methods. We synthesized of 125I-IPOS, a 125I labeled chimeric protein probe, that would visualize HIF activity. The biodistribution of 125I-IPOS in FM3A tumor-bearing mice was evaluated. Then, the intratumoral localization of this probe was observed by autoradiography, and it was compared with histopathological findings. The distribution of 125I-IPOS in tumors was imaged by a small animal SPECT/CT scanner. The obtained in vivo SPECT-CT fusion images were compared with ex vivo images of excised tumors. Fusion imaging with MRI was also examined. Results. 125I-IPOS well accumulated in FM3A tumors. The intratumoral distribution of 125I-IPOS by autoradiography was quite heterogeneous, and it partially overlapped with that of pimonidazole. High-resolution SPECT-CT fusion images successfully demonstrated the heterogeneity of 125I-IPOS distribution inside tumors. SPECT-MRI fusion images could give more detailed information about the intratumoral distribution of 125I-IPOS. Conclusion. High-resolution SPECT images successfully demonstrated heterogeneous intratumoral distribution of 125I-IPOS. SPECT-CT fusion images, more favorably SPECT-MRI fusion images, would be useful to understand the features of heterogeneous intratumoral expression of HIF activity in vivo.",
author = "Hirofumi Fujii and Masayuki Yamaguchi and Kazumasa Inoue and Yasuko Mutou and Masashi Ueda and Hideo Saji and Shinae Kizaka-Kondoh and Noriyuki Moriyama and Umeda, {Izumi O.}",
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AU - Yamaguchi, Masayuki

AU - Inoue, Kazumasa

AU - Mutou, Yasuko

AU - Ueda, Masashi

AU - Saji, Hideo

AU - Kizaka-Kondoh, Shinae

AU - Moriyama, Noriyuki

AU - Umeda, Izumi O.

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N2 - Purpose. We aimed to clearly visualize heterogeneous distribution of hypoxia-inducible factor 1 (HIF) activity in tumor tissues in vivo. Methods. We synthesized of 125I-IPOS, a 125I labeled chimeric protein probe, that would visualize HIF activity. The biodistribution of 125I-IPOS in FM3A tumor-bearing mice was evaluated. Then, the intratumoral localization of this probe was observed by autoradiography, and it was compared with histopathological findings. The distribution of 125I-IPOS in tumors was imaged by a small animal SPECT/CT scanner. The obtained in vivo SPECT-CT fusion images were compared with ex vivo images of excised tumors. Fusion imaging with MRI was also examined. Results. 125I-IPOS well accumulated in FM3A tumors. The intratumoral distribution of 125I-IPOS by autoradiography was quite heterogeneous, and it partially overlapped with that of pimonidazole. High-resolution SPECT-CT fusion images successfully demonstrated the heterogeneity of 125I-IPOS distribution inside tumors. SPECT-MRI fusion images could give more detailed information about the intratumoral distribution of 125I-IPOS. Conclusion. High-resolution SPECT images successfully demonstrated heterogeneous intratumoral distribution of 125I-IPOS. SPECT-CT fusion images, more favorably SPECT-MRI fusion images, would be useful to understand the features of heterogeneous intratumoral expression of HIF activity in vivo.

AB - Purpose. We aimed to clearly visualize heterogeneous distribution of hypoxia-inducible factor 1 (HIF) activity in tumor tissues in vivo. Methods. We synthesized of 125I-IPOS, a 125I labeled chimeric protein probe, that would visualize HIF activity. The biodistribution of 125I-IPOS in FM3A tumor-bearing mice was evaluated. Then, the intratumoral localization of this probe was observed by autoradiography, and it was compared with histopathological findings. The distribution of 125I-IPOS in tumors was imaged by a small animal SPECT/CT scanner. The obtained in vivo SPECT-CT fusion images were compared with ex vivo images of excised tumors. Fusion imaging with MRI was also examined. Results. 125I-IPOS well accumulated in FM3A tumors. The intratumoral distribution of 125I-IPOS by autoradiography was quite heterogeneous, and it partially overlapped with that of pimonidazole. High-resolution SPECT-CT fusion images successfully demonstrated the heterogeneity of 125I-IPOS distribution inside tumors. SPECT-MRI fusion images could give more detailed information about the intratumoral distribution of 125I-IPOS. Conclusion. High-resolution SPECT images successfully demonstrated heterogeneous intratumoral distribution of 125I-IPOS. SPECT-CT fusion images, more favorably SPECT-MRI fusion images, would be useful to understand the features of heterogeneous intratumoral expression of HIF activity in vivo.

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