In vivo tissue response of endodontic bio-ceramic materials

Teresita Romano, María Victoria Jammal, Keisuke Nakano, Ana García Rusco, Jorge Olmos Fassi, Silvia Kozuszko, Kiyofumi Takabatake, Hitoshi Nagatsuka, Liliana Raquel Missana

Research output: Contribution to journalArticle

Abstract

In order to evaluate the biocompatibility and mineral repair capacity, mineral trioxide aggregate (MTA), portland cement normal setting (PCn) and fast setting (PCf) and calcium hydroxide-based paste (Calen) filled silicone tube were implanted into the dorsal subcutaneous connective tissues of 25 Wistar rats. Animals were euthanized at 24, 72 hours, 7, 15 and 30 days. Implants with surrounding tissues were fixed with 10% buffer formaldehyde and processed for histological routine techniques. Slides (5 µm serial cuts) were stained with H&E and Von Kossa stains for morphological, qualitative and quantitative analysis by light microscopy. Calen showed severe and moderate inflammatory response and granuloma-tous reaction with psammoma body-like formation. PCn and MTA have similar behavior, with mild inflammatory reaction from 8% and 4%, respectively. Even though, PCn and MTA demonstrated analogous biological reaction, MTA developing thick artificial mineral precipitation (p = 0,007). All sealers demonstrated a similar inflammatory response at all time periods studied (p = 0.678).

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalJournal of Hard Tissue Biology
Volume28
Issue number1
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Endodontics
Ceramics
Ceramic materials
Portland cement
Minerals
Tissue
Calcium Hydroxide
Histological Techniques
Subcutaneous Tissue
Silicones
Ointments
Granuloma
Biocompatibility
Connective Tissue
Formaldehyde
Optical microscopy
Wistar Rats
Rats
Microscopy
Buffers

Keywords

  • Biocompatibility
  • Calcium hydroxide
  • Mineral trioxide aggregate
  • Portland cement

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biomaterials
  • Orthopedics and Sports Medicine
  • Dentistry(all)
  • Cell Biology

Cite this

Romano, T., Jammal, M. V., Nakano, K., Rusco, A. G., Fassi, J. O., Kozuszko, S., ... Missana, L. R. (2019). In vivo tissue response of endodontic bio-ceramic materials. Journal of Hard Tissue Biology, 28(1), 1-6. https://doi.org/10.2485/jhtb.28.1

In vivo tissue response of endodontic bio-ceramic materials. / Romano, Teresita; Jammal, María Victoria; Nakano, Keisuke; Rusco, Ana García; Fassi, Jorge Olmos; Kozuszko, Silvia; Takabatake, Kiyofumi; Nagatsuka, Hitoshi; Missana, Liliana Raquel.

In: Journal of Hard Tissue Biology, Vol. 28, No. 1, 01.01.2019, p. 1-6.

Research output: Contribution to journalArticle

Romano, T, Jammal, MV, Nakano, K, Rusco, AG, Fassi, JO, Kozuszko, S, Takabatake, K, Nagatsuka, H & Missana, LR 2019, 'In vivo tissue response of endodontic bio-ceramic materials', Journal of Hard Tissue Biology, vol. 28, no. 1, pp. 1-6. https://doi.org/10.2485/jhtb.28.1
Romano, Teresita ; Jammal, María Victoria ; Nakano, Keisuke ; Rusco, Ana García ; Fassi, Jorge Olmos ; Kozuszko, Silvia ; Takabatake, Kiyofumi ; Nagatsuka, Hitoshi ; Missana, Liliana Raquel. / In vivo tissue response of endodontic bio-ceramic materials. In: Journal of Hard Tissue Biology. 2019 ; Vol. 28, No. 1. pp. 1-6.
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