In vivo robustness analysis of cell division cycle genes in Saccharomyces cerevisiae.

Hisao Moriya, Yuki Shimizu-Yoshida, Hiroaki Kitano

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Intracellular biochemical parameters, such as the expression level of gene products, are considered to be optimized so that a biological system, including the parameters, works effectively. Those parameters should have some permissible range so that the systems have robustness against perturbations, such as noise in gene expression. However, little is known about the permissible range in real cells because there has been no experimental technique to test it. In this study, we developed a genetic screening method, named "genetic tug-of-war" (gTOW) that evaluates upper limit copy numbers of genes in a model eukaryote Saccharomyces cerevisiae, and we applied it for 30 cell-cycle related genes (CDC genes). The experiment provided unique quantitative data that could be used to argue the system-level properties of the cell cycle such as robustness and fragility. The data were used to evaluate the current computational model, and refinements to the model were suggested.

Original languageEnglish
JournalPLoS Genetics
Volume2
Issue number7
DOIs
Publication statusPublished - Jul 2006
Externally publishedYes

Fingerprint

cdc Genes
Saccharomyces cerevisiae
cell division
Gene Expression
Gene Dosage
gene
cell cycle
Genetic Testing
Centers for Disease Control and Prevention (U.S.)
Eukaryota
Noise
gene dosage
Cell Cycle
genes
tug
eukaryotic cells
eukaryote
Genes
screening
gene expression

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

In vivo robustness analysis of cell division cycle genes in Saccharomyces cerevisiae. / Moriya, Hisao; Shimizu-Yoshida, Yuki; Kitano, Hiroaki.

In: PLoS Genetics, Vol. 2, No. 7, 07.2006.

Research output: Contribution to journalArticle

Moriya, Hisao ; Shimizu-Yoshida, Yuki ; Kitano, Hiroaki. / In vivo robustness analysis of cell division cycle genes in Saccharomyces cerevisiae. In: PLoS Genetics. 2006 ; Vol. 2, No. 7.
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