Preliminary analysis of cells before using them in tissue engineering is mandatory. Thus, to clarify the behavior of cells in an appropriate experimental model, we evaluated the characteristic of MDPC-23 cells and KUSA/A1 cells in vitro and in vivo seeded in diffusion chamber. Our results indicated that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber. Whereas, MDPC-23 cells were odontoblast-like cells but with low ability to induce dentin formation. This study suggests that MDPC-23 cells are special cells, which possess morphological and functional character of odontoblast-like cells, expressing DSPP only in vivo with low capacity to induce mineralized dentin matrix.
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Orthopedics and Sports Medicine
- Cell Biology