In vivo multitracer analysis technique: Screening of radioactive probes for noninvasive measurement of physiological functions in experimental animals

Ken Ichiro Matsumoto, Rieko Hirunuma, Shuichi Enomoto, Kazutoyo Endo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A novel screening experiment, to find radioactive probes for non-invasive measurements of physiological functions in experimental animals, was tested using the in vivo multitracer analysis technique. The details of the efficiency of the detector settings used in the in vivo multitracer analysis technique were examined by both computer simulations and practical measurements. Multiple radioactive isotopes, i.e. multitracer, were prepared by irradiating a silver foil target with a heavy ion beam at the RIKEN ring cyclotron. After chemical separation of the silver target, the multitracer was finally dissolved in isotonic citrate buffer. The multitracer solution was intravenously injected into rats. Using a γ-ray detector equipped with a well-defined slit, the collimated γ-rays from the upper abdomen of living rats were measured. After correction of detection efficiencies, it was possible to compare the distribution of radioactive elements between two groups of rats different in body weight. The in vivo measurement showed that the tissue substantial volume of the selenium-deficient (SeD) rat liver increased compared to normal rats. The possibility of a functional estimation of tissue/blood volume for living rats was proposed based on the characteristic in vivo distribution of 74As, 83Rb and 103Ru.

Original languageEnglish
Pages (from-to)2029-2034
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Volume28
Issue number11
DOIs
Publication statusPublished - Nov 2005
Externally publishedYes

Fingerprint

Silver
Radioactive Elements
Cyclotrons
Heavy Ions
Selenium
Blood Volume
Citric Acid
Radioisotopes
Computer Simulation
Abdomen
Buffers
Body Weight
Liver

Keywords

  • Bio-trace element
  • Blood volume
  • Extracellular volume
  • Intracellular volume
  • Liver
  • Rat

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

In vivo multitracer analysis technique : Screening of radioactive probes for noninvasive measurement of physiological functions in experimental animals. / Matsumoto, Ken Ichiro; Hirunuma, Rieko; Enomoto, Shuichi; Endo, Kazutoyo.

In: Biological and Pharmaceutical Bulletin, Vol. 28, No. 11, 11.2005, p. 2029-2034.

Research output: Contribution to journalArticle

@article{c1774d6eccd34692aaa493f8a48412dc,
title = "In vivo multitracer analysis technique: Screening of radioactive probes for noninvasive measurement of physiological functions in experimental animals",
abstract = "A novel screening experiment, to find radioactive probes for non-invasive measurements of physiological functions in experimental animals, was tested using the in vivo multitracer analysis technique. The details of the efficiency of the detector settings used in the in vivo multitracer analysis technique were examined by both computer simulations and practical measurements. Multiple radioactive isotopes, i.e. multitracer, were prepared by irradiating a silver foil target with a heavy ion beam at the RIKEN ring cyclotron. After chemical separation of the silver target, the multitracer was finally dissolved in isotonic citrate buffer. The multitracer solution was intravenously injected into rats. Using a γ-ray detector equipped with a well-defined slit, the collimated γ-rays from the upper abdomen of living rats were measured. After correction of detection efficiencies, it was possible to compare the distribution of radioactive elements between two groups of rats different in body weight. The in vivo measurement showed that the tissue substantial volume of the selenium-deficient (SeD) rat liver increased compared to normal rats. The possibility of a functional estimation of tissue/blood volume for living rats was proposed based on the characteristic in vivo distribution of 74As, 83Rb and 103Ru.",
keywords = "Bio-trace element, Blood volume, Extracellular volume, Intracellular volume, Liver, Rat",
author = "Matsumoto, {Ken Ichiro} and Rieko Hirunuma and Shuichi Enomoto and Kazutoyo Endo",
year = "2005",
month = "11",
doi = "10.1248/bpb.28.2029",
language = "English",
volume = "28",
pages = "2029--2034",
journal = "Biological and Pharmaceutical Bulletin",
issn = "0918-6158",
publisher = "Pharmaceutical Society of Japan",
number = "11",

}

TY - JOUR

T1 - In vivo multitracer analysis technique

T2 - Screening of radioactive probes for noninvasive measurement of physiological functions in experimental animals

AU - Matsumoto, Ken Ichiro

AU - Hirunuma, Rieko

AU - Enomoto, Shuichi

AU - Endo, Kazutoyo

PY - 2005/11

Y1 - 2005/11

N2 - A novel screening experiment, to find radioactive probes for non-invasive measurements of physiological functions in experimental animals, was tested using the in vivo multitracer analysis technique. The details of the efficiency of the detector settings used in the in vivo multitracer analysis technique were examined by both computer simulations and practical measurements. Multiple radioactive isotopes, i.e. multitracer, were prepared by irradiating a silver foil target with a heavy ion beam at the RIKEN ring cyclotron. After chemical separation of the silver target, the multitracer was finally dissolved in isotonic citrate buffer. The multitracer solution was intravenously injected into rats. Using a γ-ray detector equipped with a well-defined slit, the collimated γ-rays from the upper abdomen of living rats were measured. After correction of detection efficiencies, it was possible to compare the distribution of radioactive elements between two groups of rats different in body weight. The in vivo measurement showed that the tissue substantial volume of the selenium-deficient (SeD) rat liver increased compared to normal rats. The possibility of a functional estimation of tissue/blood volume for living rats was proposed based on the characteristic in vivo distribution of 74As, 83Rb and 103Ru.

AB - A novel screening experiment, to find radioactive probes for non-invasive measurements of physiological functions in experimental animals, was tested using the in vivo multitracer analysis technique. The details of the efficiency of the detector settings used in the in vivo multitracer analysis technique were examined by both computer simulations and practical measurements. Multiple radioactive isotopes, i.e. multitracer, were prepared by irradiating a silver foil target with a heavy ion beam at the RIKEN ring cyclotron. After chemical separation of the silver target, the multitracer was finally dissolved in isotonic citrate buffer. The multitracer solution was intravenously injected into rats. Using a γ-ray detector equipped with a well-defined slit, the collimated γ-rays from the upper abdomen of living rats were measured. After correction of detection efficiencies, it was possible to compare the distribution of radioactive elements between two groups of rats different in body weight. The in vivo measurement showed that the tissue substantial volume of the selenium-deficient (SeD) rat liver increased compared to normal rats. The possibility of a functional estimation of tissue/blood volume for living rats was proposed based on the characteristic in vivo distribution of 74As, 83Rb and 103Ru.

KW - Bio-trace element

KW - Blood volume

KW - Extracellular volume

KW - Intracellular volume

KW - Liver

KW - Rat

UR - http://www.scopus.com/inward/record.url?scp=27744558548&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27744558548&partnerID=8YFLogxK

U2 - 10.1248/bpb.28.2029

DO - 10.1248/bpb.28.2029

M3 - Article

C2 - 16272684

AN - SCOPUS:27744558548

VL - 28

SP - 2029

EP - 2034

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

SN - 0918-6158

IS - 11

ER -