In vivo-like cell-cycle phase distribution of cancer cells in Gelfoam® histoculture observed in real time by FUCCI imaging

Robert M. Hoffman, Shuya Yano

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

FUCCI color codes cells as they express different color fluorescent proteins as they go through phases of the cell cycle. The cell cycle phase distribution of cancer cells in Gelfoam® histoculture was similar to in vivo tumors, whereby only the surface cells proliferate and interior cells are quiescent in G0/G1. In contrast, in 2D cancer-cell culture, most of the cells are always cycling. The cancer cells responded similarly to toxic chemotherapy in Gelfoam® culture as in vivo; as such, therapy is cell cycle dependent. In 2D culture, cancer cells were much more chemosensitive. These results indicate why the drug response pattern of tumors in Gelfoam® histoculture reflects what is observed in vivo.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages109-123
Number of pages15
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1760
ISSN (Print)1064-3745

Keywords

  • 3D culture
  • Cell cycle
  • Chemotherapy
  • Color-coding
  • FUCCI (fluorescence ubiquitination cell cycle indicator)
  • Gelfoam histoculture
  • Imaging

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Hoffman, R. M., & Yano, S. (2018). In vivo-like cell-cycle phase distribution of cancer cells in Gelfoam® histoculture observed in real time by FUCCI imaging. In Methods in Molecular Biology (pp. 109-123). (Methods in Molecular Biology; Vol. 1760). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7745-1_12