In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle

Koichi Oishi, Yoshitaka Miyamoto, Hiroaki Saito, Katsutoshi Murase, Kenji Ono, Makoto Sawada, Masami Watanabe, Yasufumi Noguchi, Toshiyoshi Fujiwara, Shuji Hayashi, Hirofumi Noguchi

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14 Citations (Scopus)

Abstract

To monitor pancreatic islet transplantation efficiency, reliable noninvasive imaging methods, such as magnetic resonance imaging (MRI) are needed. Although an efficient uptake of MRI contrast agent is required for islet cell labeling, commercially-available magnetic nanoparticles are not efficiently transduced into cells. We herein report the in vivo detection of transplanted islets labeled with a novel cationic nanoparticle that allowed for noninvasive monitoring of islet grafts in diabetic mice in real time. The positively-charged nanoparticles were transduced into a β-cell line, MIN6 cells, and into isolated islets for 1 hr. MRI showed a marked decrease in the signal intensity on T1- and T2-weighted images at the implantation site of the labeled MIN 6 cells or islets in the left kidneys of mice. These data suggest that the novel positively-charged nanoparticle could be useful to detect and monitor islet engraftment, which would greatly aid in the clinical management of islet transplant patients.

Original languageEnglish
Article numbere57046
JournalPloS one
Volume8
Issue number2
DOIs
Publication statusPublished - Feb 22 2013

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Oishi, K., Miyamoto, Y., Saito, H., Murase, K., Ono, K., Sawada, M., Watanabe, M., Noguchi, Y., Fujiwara, T., Hayashi, S., & Noguchi, H. (2013). In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle. PloS one, 8(2), [e57046]. https://doi.org/10.1371/journal.pone.0057046