TY - JOUR
T1 - In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle
AU - Oishi, Koichi
AU - Miyamoto, Yoshitaka
AU - Saito, Hiroaki
AU - Murase, Katsutoshi
AU - Ono, Kenji
AU - Sawada, Makoto
AU - Watanabe, Masami
AU - Noguchi, Yasufumi
AU - Fujiwara, Toshiyoshi
AU - Hayashi, Shuji
AU - Noguchi, Hirofumi
PY - 2013/2/22
Y1 - 2013/2/22
N2 - To monitor pancreatic islet transplantation efficiency, reliable noninvasive imaging methods, such as magnetic resonance imaging (MRI) are needed. Although an efficient uptake of MRI contrast agent is required for islet cell labeling, commercially-available magnetic nanoparticles are not efficiently transduced into cells. We herein report the in vivo detection of transplanted islets labeled with a novel cationic nanoparticle that allowed for noninvasive monitoring of islet grafts in diabetic mice in real time. The positively-charged nanoparticles were transduced into a β-cell line, MIN6 cells, and into isolated islets for 1 hr. MRI showed a marked decrease in the signal intensity on T1- and T2-weighted images at the implantation site of the labeled MIN 6 cells or islets in the left kidneys of mice. These data suggest that the novel positively-charged nanoparticle could be useful to detect and monitor islet engraftment, which would greatly aid in the clinical management of islet transplant patients.
AB - To monitor pancreatic islet transplantation efficiency, reliable noninvasive imaging methods, such as magnetic resonance imaging (MRI) are needed. Although an efficient uptake of MRI contrast agent is required for islet cell labeling, commercially-available magnetic nanoparticles are not efficiently transduced into cells. We herein report the in vivo detection of transplanted islets labeled with a novel cationic nanoparticle that allowed for noninvasive monitoring of islet grafts in diabetic mice in real time. The positively-charged nanoparticles were transduced into a β-cell line, MIN6 cells, and into isolated islets for 1 hr. MRI showed a marked decrease in the signal intensity on T1- and T2-weighted images at the implantation site of the labeled MIN 6 cells or islets in the left kidneys of mice. These data suggest that the novel positively-charged nanoparticle could be useful to detect and monitor islet engraftment, which would greatly aid in the clinical management of islet transplant patients.
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U2 - 10.1371/journal.pone.0057046
DO - 10.1371/journal.pone.0057046
M3 - Article
C2 - 23451139
AN - SCOPUS:84874334905
VL - 8
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 2
M1 - e57046
ER -