In vivo imaging of human cancer with telomerase-specific replication-selective adenovirus

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Tumor-specific replication-competent viruses represent a novel approach for the treatment of neoplastic disease. These vectors can be used to directly label tumor cells in vivo, as they are designed to selectively replicate within such cells. To target cancer cells, there is a need for tissue- or cell-specific promoters that are expressed in diverse tumor types and are silent in normal cells. Telomerase activation is considered to be a critical step in carcinogenesis through the maintenance of telomeres, and its activity is closely correlated with human telomerase reverse transcriptase (hTERT) expression. We constructed a green fluorescent protein (GFP)-expressing attenuated adenovirus-5 vector, in which the hTERT promoter regulates viral replication (TelomeScan, OBP-401). We used TelomeScan to establish a new approach for visualizing metastatic or disseminated human tumors in vivo. Visualization is achieved via illumination with an excitation lamp under a three-chip color-cooled charged-coupled device camera following injection of TelomeScan into primary tumors or tumor-disseminated cavities. TelomeScan infection increases the signal-to-background ratio as a tumor-specific probe, because the fluorescent signals are only amplified in tumor cells by viral replication. This technology is adaptable to detect tumor metastasis and/or dissemination in vivo as a preclinical model of surgical navigation.

Original languageEnglish
Title of host publicationIn Vivo Cellular Imaging Using Fluorescent Proteins
Subtitle of host publicationMethods and Protocols
EditorsRobert M. Hoffman
Pages129-139
Number of pages11
DOIs
Publication statusPublished - Dec 1 2012

Publication series

NameMethods in Molecular Biology
Volume872
ISSN (Print)1064-3745

Keywords

  • Adenovirus
  • GFP
  • Metastasis
  • Surgical navigation
  • Telomerase

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Fujiwara, T. (2012). In vivo imaging of human cancer with telomerase-specific replication-selective adenovirus. In R. M. Hoffman (Ed.), In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols (pp. 129-139). (Methods in Molecular Biology; Vol. 872). https://doi.org/10.1007/978-1-61779-797-2_9