In vivo gene transfer into the adult mammalian central nervous system by continuous injection of plasmid DNA-cationic liposome complex

Takashi Imaoka, Isao Date, Takashi Ohmoto, Tatsuji Yasuda, Masaaki Tsuda

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Previously reported methods of liposome-mediated direct in vivo gene transfer have been inefficient, especially when performed with highly differentiated, quiescent cells of the adult mammalian central nervous system (CNS). We have therefore improved these procedures based upon a novel concept. Following continuous injection of plasmid DNA-cationic liposome complex which contained a reporter gene encoding E. coli β-galactosidase into the striatum of adult rats, the expression of transgene was dramatically elevated without any adverse effects. This new technique may enable a wide application of liposome-mediated gene transfer technology not only to basic analysis of gene functions in the brain but also for clinical treatment of certain CNS disorders.

Original languageEnglish
Pages (from-to)119-128
Number of pages10
JournalBrain Research
Volume780
Issue number1
DOIs
Publication statusPublished - Jan 5 1998

Keywords

  • Adult mammalian CNS
  • Continuous injection
  • Gene transfer
  • Plasmid DNA-cationic liposome complex

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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