In Vivo evaluation of adipogenic induction in fibrous and honeycomb-structured atelocollagen scaffolds

Andrea P. Rodríguez, Betiana Felice, María A. Sánchez, Hidetsugu Tsujigiwa, Carmelo J. Felice, Hitoshi Nagatsuka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Nowadays, soft tissue restoration techniques are mainly focused on volume regeneration instead of function recovering. So far, autologous fat transplant has been the most popular method although its multiple reported problems like volume and function loss. Adipose tissue engineering therefore emerges as a solution for development of biological substitutes for soft tissue which promotes not only volume regeneration but also function restoration with minimal consequences. Here we tested fibrous-structured atelocollagen (FSA) scaffolds and honeycomb atelocollagen (HCA) scaffolds for their ability to induce adipogenesis in vivo. Implants were subjected to histological and immunohistochemical assessment after 1, 2, and 4 weeks of implantation. Our studies showed that FSA scaffolds induced in vivo a markedly adipogenic response, whereas an acute inflammatory process was observed at HCA scaffolds without tissue regeneration detected within them. Our histological findings concerning FSA scaffolds clearly showed the presence of adipose-like tissue surprisingly composed by a mixture of brown-like and white-like adipocytes at week 2 whereas only white-like adipocytes at week 4. Subsequent positive Pax7 immunostaining at weeks 1 and 2 suggested the existence of a common myogenic progenitor shared by brown-like and white-like adipocytes observed. Then, in this work we present FSA scaffolds as a promising structure for brown and white adipose tissue engineering.

Original languageEnglish
Pages (from-to)125-130
Number of pages6
JournalMaterials Science and Engineering C
Volume63
DOIs
Publication statusPublished - Jun 1 2016

Fingerprint

adipose tissues
regeneration
Scaffolds
induction
tissue engineering
restoration
evaluation
fats
Tissue
Tissue engineering
Restoration
implantation
substitutes
Tissue regeneration
Transplants
Oils and fats
atelocollagen
Fats

Keywords

  • Adipose tissue engineering
  • Atelocollagen
  • In vivo
  • Myogenic origin
  • White adipocytes

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

In Vivo evaluation of adipogenic induction in fibrous and honeycomb-structured atelocollagen scaffolds. / Rodríguez, Andrea P.; Felice, Betiana; Sánchez, María A.; Tsujigiwa, Hidetsugu; Felice, Carmelo J.; Nagatsuka, Hitoshi.

In: Materials Science and Engineering C, Vol. 63, 01.06.2016, p. 125-130.

Research output: Contribution to journalArticle

Rodríguez, Andrea P. ; Felice, Betiana ; Sánchez, María A. ; Tsujigiwa, Hidetsugu ; Felice, Carmelo J. ; Nagatsuka, Hitoshi. / In Vivo evaluation of adipogenic induction in fibrous and honeycomb-structured atelocollagen scaffolds. In: Materials Science and Engineering C. 2016 ; Vol. 63. pp. 125-130.
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