TY - JOUR
T1 - In Vivo evaluation of adipogenic induction in fibrous and honeycomb-structured atelocollagen scaffolds
AU - Rodríguez, Andrea P.
AU - Felice, Betiana
AU - Sánchez, María A.
AU - Tsujigiwa, Hidetsugu
AU - Felice, Carmelo J.
AU - Nagatsuka, Hitoshi
N1 - Funding Information:
Research conduction of this study was supported by the Japan Society for the Promotion of Science ( 19/07217 , Japan). Article preparation was financed by the National Agency for Science and Technology ( PICT 2009 n°87 , Argentina).
Publisher Copyright:
© 2016 Elsevier B.V. All Rights Reserved.
PY - 2016/6/1
Y1 - 2016/6/1
N2 - Nowadays, soft tissue restoration techniques are mainly focused on volume regeneration instead of function recovering. So far, autologous fat transplant has been the most popular method although its multiple reported problems like volume and function loss. Adipose tissue engineering therefore emerges as a solution for development of biological substitutes for soft tissue which promotes not only volume regeneration but also function restoration with minimal consequences. Here we tested fibrous-structured atelocollagen (FSA) scaffolds and honeycomb atelocollagen (HCA) scaffolds for their ability to induce adipogenesis in vivo. Implants were subjected to histological and immunohistochemical assessment after 1, 2, and 4 weeks of implantation. Our studies showed that FSA scaffolds induced in vivo a markedly adipogenic response, whereas an acute inflammatory process was observed at HCA scaffolds without tissue regeneration detected within them. Our histological findings concerning FSA scaffolds clearly showed the presence of adipose-like tissue surprisingly composed by a mixture of brown-like and white-like adipocytes at week 2 whereas only white-like adipocytes at week 4. Subsequent positive Pax7 immunostaining at weeks 1 and 2 suggested the existence of a common myogenic progenitor shared by brown-like and white-like adipocytes observed. Then, in this work we present FSA scaffolds as a promising structure for brown and white adipose tissue engineering.
AB - Nowadays, soft tissue restoration techniques are mainly focused on volume regeneration instead of function recovering. So far, autologous fat transplant has been the most popular method although its multiple reported problems like volume and function loss. Adipose tissue engineering therefore emerges as a solution for development of biological substitutes for soft tissue which promotes not only volume regeneration but also function restoration with minimal consequences. Here we tested fibrous-structured atelocollagen (FSA) scaffolds and honeycomb atelocollagen (HCA) scaffolds for their ability to induce adipogenesis in vivo. Implants were subjected to histological and immunohistochemical assessment after 1, 2, and 4 weeks of implantation. Our studies showed that FSA scaffolds induced in vivo a markedly adipogenic response, whereas an acute inflammatory process was observed at HCA scaffolds without tissue regeneration detected within them. Our histological findings concerning FSA scaffolds clearly showed the presence of adipose-like tissue surprisingly composed by a mixture of brown-like and white-like adipocytes at week 2 whereas only white-like adipocytes at week 4. Subsequent positive Pax7 immunostaining at weeks 1 and 2 suggested the existence of a common myogenic progenitor shared by brown-like and white-like adipocytes observed. Then, in this work we present FSA scaffolds as a promising structure for brown and white adipose tissue engineering.
KW - Adipose tissue engineering
KW - Atelocollagen
KW - In vivo
KW - Myogenic origin
KW - White adipocytes
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U2 - 10.1016/j.msec.2016.02.055
DO - 10.1016/j.msec.2016.02.055
M3 - Article
C2 - 27040203
AN - SCOPUS:84959376959
SN - 0928-4931
VL - 63
SP - 125
EP - 130
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
ER -