In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharide-treated mice

Tetsuhiko Yoshimura, Hidekatsu Yokoyama, Satoshi Fujii, Fusako Takayama, Kazuo Oikawa, Hitoshi Kamada

Research output: Contribution to journalArticle

194 Citations (Scopus)

Abstract

Nitric oxide (NO), a simple diatomic free radical, is known to play a critical physiological role in diverse organisms. An iron complex, with N- (dithiocarboxy)sarcosine (Fe-DTCS), has a high affinity for endogenous NO and can trap, stabilize, and accumulate it. The stable NO adduct thus formed is detectable at room temperature with electron paramagnetic resonance (EPR) spectrometry. We report in vivo EPR imaging of endogenous NO, trapped by an Fe-DTCS complex, in the abdomen of a live mouse. To our knowledge, this is the first report on EPR imaging of endogenous free radicals produced in vivo. This EPR imaging method will be useful for the noninvasive investigation of the spatial distribution of NO in pathologic organs or tissues.

Original languageEnglish
Pages (from-to)992-994
Number of pages3
JournalNature Biotechnology
Volume14
Issue number8
Publication statusPublished - Aug 1996
Externally publishedYes

Fingerprint

Nitric oxide
Electron Spin Resonance Spectroscopy
Paramagnetic resonance
Lipopolysaccharides
Nitric Oxide
Imaging techniques
Free radicals
Free Radicals
Abdomen
Spectrometry
Spatial distribution
Spectrum Analysis
Iron
Tissue
Temperature
(N-dithiocarboxysarcosine)iron(III)

Keywords

  • EPR imaging
  • lipopolysaccharide
  • N- (dithiocarboxy)sarcosine
  • nitric oxide
  • spin-trapping

ASJC Scopus subject areas

  • Microbiology

Cite this

Yoshimura, T., Yokoyama, H., Fujii, S., Takayama, F., Oikawa, K., & Kamada, H. (1996). In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharide-treated mice. Nature Biotechnology, 14(8), 992-994.

In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharide-treated mice. / Yoshimura, Tetsuhiko; Yokoyama, Hidekatsu; Fujii, Satoshi; Takayama, Fusako; Oikawa, Kazuo; Kamada, Hitoshi.

In: Nature Biotechnology, Vol. 14, No. 8, 08.1996, p. 992-994.

Research output: Contribution to journalArticle

Yoshimura, T, Yokoyama, H, Fujii, S, Takayama, F, Oikawa, K & Kamada, H 1996, 'In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharide-treated mice', Nature Biotechnology, vol. 14, no. 8, pp. 992-994.
Yoshimura, Tetsuhiko ; Yokoyama, Hidekatsu ; Fujii, Satoshi ; Takayama, Fusako ; Oikawa, Kazuo ; Kamada, Hitoshi. / In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharide-treated mice. In: Nature Biotechnology. 1996 ; Vol. 14, No. 8. pp. 992-994.
@article{c4e0cf27049b4722a195a1e135812441,
title = "In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharide-treated mice",
abstract = "Nitric oxide (NO), a simple diatomic free radical, is known to play a critical physiological role in diverse organisms. An iron complex, with N- (dithiocarboxy)sarcosine (Fe-DTCS), has a high affinity for endogenous NO and can trap, stabilize, and accumulate it. The stable NO adduct thus formed is detectable at room temperature with electron paramagnetic resonance (EPR) spectrometry. We report in vivo EPR imaging of endogenous NO, trapped by an Fe-DTCS complex, in the abdomen of a live mouse. To our knowledge, this is the first report on EPR imaging of endogenous free radicals produced in vivo. This EPR imaging method will be useful for the noninvasive investigation of the spatial distribution of NO in pathologic organs or tissues.",
keywords = "EPR imaging, lipopolysaccharide, N- (dithiocarboxy)sarcosine, nitric oxide, spin-trapping",
author = "Tetsuhiko Yoshimura and Hidekatsu Yokoyama and Satoshi Fujii and Fusako Takayama and Kazuo Oikawa and Hitoshi Kamada",
year = "1996",
month = "8",
language = "English",
volume = "14",
pages = "992--994",
journal = "Biotechnology",
issn = "0733-222X",
publisher = "Nature Publishing Group",
number = "8",

}

TY - JOUR

T1 - In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharide-treated mice

AU - Yoshimura, Tetsuhiko

AU - Yokoyama, Hidekatsu

AU - Fujii, Satoshi

AU - Takayama, Fusako

AU - Oikawa, Kazuo

AU - Kamada, Hitoshi

PY - 1996/8

Y1 - 1996/8

N2 - Nitric oxide (NO), a simple diatomic free radical, is known to play a critical physiological role in diverse organisms. An iron complex, with N- (dithiocarboxy)sarcosine (Fe-DTCS), has a high affinity for endogenous NO and can trap, stabilize, and accumulate it. The stable NO adduct thus formed is detectable at room temperature with electron paramagnetic resonance (EPR) spectrometry. We report in vivo EPR imaging of endogenous NO, trapped by an Fe-DTCS complex, in the abdomen of a live mouse. To our knowledge, this is the first report on EPR imaging of endogenous free radicals produced in vivo. This EPR imaging method will be useful for the noninvasive investigation of the spatial distribution of NO in pathologic organs or tissues.

AB - Nitric oxide (NO), a simple diatomic free radical, is known to play a critical physiological role in diverse organisms. An iron complex, with N- (dithiocarboxy)sarcosine (Fe-DTCS), has a high affinity for endogenous NO and can trap, stabilize, and accumulate it. The stable NO adduct thus formed is detectable at room temperature with electron paramagnetic resonance (EPR) spectrometry. We report in vivo EPR imaging of endogenous NO, trapped by an Fe-DTCS complex, in the abdomen of a live mouse. To our knowledge, this is the first report on EPR imaging of endogenous free radicals produced in vivo. This EPR imaging method will be useful for the noninvasive investigation of the spatial distribution of NO in pathologic organs or tissues.

KW - EPR imaging

KW - lipopolysaccharide

KW - N- (dithiocarboxy)sarcosine

KW - nitric oxide

KW - spin-trapping

UR - http://www.scopus.com/inward/record.url?scp=0029818087&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029818087&partnerID=8YFLogxK

M3 - Article

C2 - 9631037

AN - SCOPUS:0029818087

VL - 14

SP - 992

EP - 994

JO - Biotechnology

JF - Biotechnology

SN - 0733-222X

IS - 8

ER -