In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharide-treated mice

Tetsuhiko Yoshimura; Hidekatsu Yokoyama; Satoshi Fujii; Fusako Takayama; Kazuo Oikawa; Hitoshi Kamada

doi:10.1038/nbt0896-992

In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharide-treated mice

Tetsuhiko Yoshimura, Hidekatsu Yokoyama, Satoshi Fujii, Fusako Takayama, Kazuo Oikawa, Hitoshi Kamada

Research output: Contribution to journal › Article › peer-review

211 Citations (Scopus)

Abstract

Nitric oxide (NO), a simple diatomic free radical, is known to play a critical physiological role in diverse organisms. An iron complex, with N- (dithiocarboxy)sarcosine (Fe-DTCS), has a high affinity for endogenous NO and can trap, stabilize, and accumulate it. The stable NO adduct thus formed is detectable at room temperature with electron paramagnetic resonance (EPR) spectrometry. We report in vivo EPR imaging of endogenous NO, trapped by an Fe-DTCS complex, in the abdomen of a live mouse. To our knowledge, this is the first report on EPR imaging of endogenous free radicals produced in vivo. This EPR imaging method will be useful for the noninvasive investigation of the spatial distribution of NO in pathologic organs or tissues.

Original language	English
Pages (from-to)	992-994
Number of pages	3
Journal	Nature Biotechnology
Volume	14
Issue number	8
DOIs	https://doi.org/10.1038/nbt0896-992
Publication status	Published - Aug 1996
Externally published	Yes

Keywords

EPR imaging
N- (dithiocarboxy)sarcosine
lipopolysaccharide
nitric oxide
spin-trapping

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Molecular Medicine
Biomedical Engineering

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10.1038/nbt0896-992

Cite this

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title = "In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharide-treated mice",

abstract = "Nitric oxide (NO), a simple diatomic free radical, is known to play a critical physiological role in diverse organisms. An iron complex, with N- (dithiocarboxy)sarcosine (Fe-DTCS), has a high affinity for endogenous NO and can trap, stabilize, and accumulate it. The stable NO adduct thus formed is detectable at room temperature with electron paramagnetic resonance (EPR) spectrometry. We report in vivo EPR imaging of endogenous NO, trapped by an Fe-DTCS complex, in the abdomen of a live mouse. To our knowledge, this is the first report on EPR imaging of endogenous free radicals produced in vivo. This EPR imaging method will be useful for the noninvasive investigation of the spatial distribution of NO in pathologic organs or tissues.",

keywords = "EPR imaging, N- (dithiocarboxy)sarcosine, lipopolysaccharide, nitric oxide, spin-trapping",

author = "Tetsuhiko Yoshimura and Hidekatsu Yokoyama and Satoshi Fujii and Fusako Takayama and Kazuo Oikawa and Hitoshi Kamada",

year = "1996",

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T1 - In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharide-treated mice

AU - Yoshimura, Tetsuhiko

AU - Yokoyama, Hidekatsu

AU - Fujii, Satoshi

AU - Takayama, Fusako

AU - Oikawa, Kazuo

AU - Kamada, Hitoshi

PY - 1996/8

Y1 - 1996/8

N2 - Nitric oxide (NO), a simple diatomic free radical, is known to play a critical physiological role in diverse organisms. An iron complex, with N- (dithiocarboxy)sarcosine (Fe-DTCS), has a high affinity for endogenous NO and can trap, stabilize, and accumulate it. The stable NO adduct thus formed is detectable at room temperature with electron paramagnetic resonance (EPR) spectrometry. We report in vivo EPR imaging of endogenous NO, trapped by an Fe-DTCS complex, in the abdomen of a live mouse. To our knowledge, this is the first report on EPR imaging of endogenous free radicals produced in vivo. This EPR imaging method will be useful for the noninvasive investigation of the spatial distribution of NO in pathologic organs or tissues.

AB - Nitric oxide (NO), a simple diatomic free radical, is known to play a critical physiological role in diverse organisms. An iron complex, with N- (dithiocarboxy)sarcosine (Fe-DTCS), has a high affinity for endogenous NO and can trap, stabilize, and accumulate it. The stable NO adduct thus formed is detectable at room temperature with electron paramagnetic resonance (EPR) spectrometry. We report in vivo EPR imaging of endogenous NO, trapped by an Fe-DTCS complex, in the abdomen of a live mouse. To our knowledge, this is the first report on EPR imaging of endogenous free radicals produced in vivo. This EPR imaging method will be useful for the noninvasive investigation of the spatial distribution of NO in pathologic organs or tissues.

KW - EPR imaging

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KW - lipopolysaccharide

KW - nitric oxide

KW - spin-trapping

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In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharide-treated mice

Abstract

Keywords

ASJC Scopus subject areas

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