In vivo effects of thyroid hormone, corticosteroids and prolactin on cell proliferation and apoptosis in the anterior intestine of the euryhaline mudskipper (Periophthalmus modestus)

Hideya Takahashi, Akiyoshi Takahashi, Tatsuya Sakamoto

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We have previously shown that anterior intestinal epithelium of the euryhaline mudskipper (Periophthalmus modestus) undergoes apoptosis during seawater (SW) acclimation, whereas elevated cell proliferation was observed in freshwater (FW)-acclimated fish. To understand the possible endocrine regulation of the gastrointestinal cell turnover during salinity acclimation, we examined the ratios of apoptotic and proliferating cells in the anterior intestine of one-third SW-acclimated mudskipper treated with triiodothyronine (T3), cortisol, 11-deoxycorticosterone (DOC, the putative teleostean mineralocorticoid), or prolactin (PRL). In situ nick end labeling of genomic DNA (TUNEL) and immunohistochemistry of proliferating cells nuclear antigen (PCNA) were used as indicators of apoptosis and cell proliferations, respectively. Cortisol significantly elevated apoptosis (P <0.05) in the epithelia and connective tissues and also stimulated the epithelial cell proliferation (P <0.05). PRL induced epithelial cell proliferation (P <0.05), but did not affect apoptotic status of the intestinal epithelium. Neither T3 nor DOC had any impact on cell proliferation or apoptosis. Together, our results suggest a role for cortisol and PRL in the regulation of anterior intestinal epithelial turnover during salinity acclimation in this species.

Original languageEnglish
Pages (from-to)1873-1880
Number of pages8
JournalLife Sciences
Volume79
Issue number19
DOIs
Publication statusPublished - Oct 4 2006

Fingerprint

Cell proliferation
Thyroid Hormones
Prolactin
Intestines
Adrenal Cortex Hormones
Cell Proliferation
Apoptosis
Acclimatization
Hydrocortisone
Salinity
In Situ Nick-End Labeling
Seawater
Intestinal Mucosa
Epithelial Cells
Enteroendocrine Cells
Desoxycorticosterone
Mineralocorticoids
Proliferating Cell Nuclear Antigen
Triiodothyronine
Fresh Water

Keywords

  • Apoptosis
  • Cell proliferation
  • Cortisol
  • Fish
  • Mineralocorticoid
  • Osmoregulation
  • Prolactin

ASJC Scopus subject areas

  • Pharmacology

Cite this

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abstract = "We have previously shown that anterior intestinal epithelium of the euryhaline mudskipper (Periophthalmus modestus) undergoes apoptosis during seawater (SW) acclimation, whereas elevated cell proliferation was observed in freshwater (FW)-acclimated fish. To understand the possible endocrine regulation of the gastrointestinal cell turnover during salinity acclimation, we examined the ratios of apoptotic and proliferating cells in the anterior intestine of one-third SW-acclimated mudskipper treated with triiodothyronine (T3), cortisol, 11-deoxycorticosterone (DOC, the putative teleostean mineralocorticoid), or prolactin (PRL). In situ nick end labeling of genomic DNA (TUNEL) and immunohistochemistry of proliferating cells nuclear antigen (PCNA) were used as indicators of apoptosis and cell proliferations, respectively. Cortisol significantly elevated apoptosis (P <0.05) in the epithelia and connective tissues and also stimulated the epithelial cell proliferation (P <0.05). PRL induced epithelial cell proliferation (P <0.05), but did not affect apoptotic status of the intestinal epithelium. Neither T3 nor DOC had any impact on cell proliferation or apoptosis. Together, our results suggest a role for cortisol and PRL in the regulation of anterior intestinal epithelial turnover during salinity acclimation in this species.",
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T1 - In vivo effects of thyroid hormone, corticosteroids and prolactin on cell proliferation and apoptosis in the anterior intestine of the euryhaline mudskipper (Periophthalmus modestus)

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AU - Takahashi, Akiyoshi

AU - Sakamoto, Tatsuya

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