In vivo direct reprogramming of glial linage to mature neurons after cerebral ischemia

Toru Yamashita, Jingwei Shang, Yumiko Nakano, Ryuta Morihara, Kota Sato, Mami Takemoto, Nozomi Hishikawa, Yasuyuki Ohta, Koji Abe

Research output: Contribution to journalArticle

Abstract

The therapeutic effect of in vivo direct reprogramming on ischemic stroke has not been evaluated. In the present study, a retroviral solution (1.5–2.0 × 107 /ul) of mock pMX-GFP (n = 13) or pMX-Ascl1/Sox2/NeuroD1 (ASN) (n = 14) was directly injected into the ipsilateral striatum and cortex 3 days after 30 min of transient cerebral ischemia. The reprogrammed cells first expressed neuronal progenitor marker Dcx 7 and 21 days after viral injection, then expressed mature neuronal marker NeuN. This was accompanied by morphological changes, including long processes and synapse-like structures, 49 days after viral injection. Meanwhile, therapeutic improvement was not detected both in clinical scores or infarct volume. The present study provides a future novel self-repair strategy for ischemic stroke with beneficial modifications of the inducer-suppressor balance.

Original languageEnglish
Article number10956
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2019

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Brain Ischemia
Neuroglia
Stroke
Neurons
Injections
Transient Ischemic Attack
Therapeutic Uses
Synapses
Therapeutics

ASJC Scopus subject areas

  • General

Cite this

In vivo direct reprogramming of glial linage to mature neurons after cerebral ischemia. / Yamashita, Toru; Shang, Jingwei; Nakano, Yumiko; Morihara, Ryuta; Sato, Kota; Takemoto, Mami; Hishikawa, Nozomi; Ohta, Yasuyuki; Abe, Koji.

In: Scientific reports, Vol. 9, No. 1, 10956, 01.12.2019.

Research output: Contribution to journalArticle

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