In vivo binding site of pemphigus vulgaris antibodies and their fate during acantholysis

K. Iwatsuki, M. Takigawa, S. Imaizumi, M. Yamada

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Ultrastructural localization of pemphigus vulgaris antigen-antibody complexes and their fate during acantholysis were studied in epidermal sheets obtained from the area surrounding the bullae and in acantholytic cells in blister fluid. The distribution of pemphigus vulgaris antibodies already bound to the keratinocytes in early acantholytic lesions was detected with ferritin-conjugated goat antihuman IgG. Ferritin particles were observed on the surface of keratinocytes with particular affinity for desmosomal structures. The acantholytic cells in the blister fluid bound only a small number of ferritin particles on their surface. During incubation at 37°C, pemphigus vulgaris antigen-antibody complexes on the surface of separated desmosomes were internalized and recognized in cytoplasmic vesicles. Endocytosis of separated desmosomes also was observed in vivo when freshly obtained epidermal sheets were immediately processed for routine electron microscopic study. These findings suggest that pemphigus vulgaris antibodies are densely located on desmosomes and that the antigen-antibody complexes, together with other serum proteins on the keratinocyte surface, are internalized by a process of endocytosis.

Original languageEnglish
Pages (from-to)578-582
Number of pages5
JournalJournal of the American Academy of Dermatology
Volume20
Issue number4
Publication statusPublished - 1989
Externally publishedYes

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Acantholysis
Pemphigus
Desmosomes
Blister
Ferritins
Antigen-Antibody Complex
Binding Sites
Keratinocytes
Antibodies
Endocytosis
Cytoplasmic Vesicles
Goats
Blood Proteins
Immunoglobulin G
Electrons

ASJC Scopus subject areas

  • Dermatology

Cite this

In vivo binding site of pemphigus vulgaris antibodies and their fate during acantholysis. / Iwatsuki, K.; Takigawa, M.; Imaizumi, S.; Yamada, M.

In: Journal of the American Academy of Dermatology, Vol. 20, No. 4, 1989, p. 578-582.

Research output: Contribution to journalArticle

Iwatsuki, K, Takigawa, M, Imaizumi, S & Yamada, M 1989, 'In vivo binding site of pemphigus vulgaris antibodies and their fate during acantholysis', Journal of the American Academy of Dermatology, vol. 20, no. 4, pp. 578-582.
Iwatsuki, K. ; Takigawa, M. ; Imaizumi, S. ; Yamada, M. / In vivo binding site of pemphigus vulgaris antibodies and their fate during acantholysis. In: Journal of the American Academy of Dermatology. 1989 ; Vol. 20, No. 4. pp. 578-582.
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