In vitro transfection with and expression of CCN family of genes

Danilo Janune, Masaharu Takigawa

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The ability to engineer cells to express a protein of interest in an inducible manner and stably for a long period is a valuable tool in molecular biology and also one that holds promise for regenerative medicine in the future. CCN proteins have been suggested to be involved in tissue regeneration. In this chapter, we describe an in vitro method for stable and inducible expression of CCN protein in a chondroprogenitor cell line and in chondrocytes in primary culture that does not involve the use of any viral vector.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages107-113
Number of pages7
DOIs
Publication statusPublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1489
ISSN (Print)1064-3745

Keywords

  • CCN
  • Expression
  • PiggyBac
  • Stable
  • Transposon

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Janune, D., & Takigawa, M. (2017). In vitro transfection with and expression of CCN family of genes. In Methods in Molecular Biology (pp. 107-113). (Methods in Molecular Biology; Vol. 1489). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6430-7_11