TY - JOUR
T1 - In vitro substrate phosphorylation by Ca2+/calmodulin-dependent protein kinase kinase using guanosine-5′-triphosphate as a phosphate donor
AU - Yurimoto, Saki
AU - Fujimoto, Tomohito
AU - Magari, Masaki
AU - Kanayama, Naoki
AU - Kobayashi, Ryoji
AU - Tokumitsu, Hiroshi
PY - 2012
Y1 - 2012
N2 - Background: Ca2+/calmodulin-dependent protein kinase kinase (CaMKK) phosphorylates and activates particular downstream protein kinases - including CaMKI, CaMKIV, and AMPK - to stimulate multiple Ca2+-signal transduction pathways. To identify previously unidentified CaMKK substrates, we used various nucleotides as phosphate donors to develop and characterize an in vitro phosphorylation assay for CaMKK. Results: Here, we found that the recombinant CaMKK isoforms were capable of utilizing Mg-GTP as a phosphate donor to phosphorylate the Thr residue in the activation-loop of CaMKIα (Thr177) and of AMPK (Thr172) in vitro. Kinetic analysis indicated that the K m values of CaMKK isoforms for GTP (400-500 μM) were significantly higher than those for ATP (∼15 μM), and a 2- to 4-fold decrease in V max was observed with GTP. We also confirmed that an ATP competitive CaMKK inhibitor, STO-609, also competes with GTP to inhibit the activities of CaMKK isoforms. In addition, to detect enhanced CaMKI phosphorylation in brain extracts with Mg-GTP and recombinant CaMKKs, we found potential CaMKK substrates of ∼45 kDa and ∼35 kDa whose Ca 2+/CaM-induced phosphorylation was inhibited by STO-609. Conclusions: These results indicated that screens that use STO-609 as a CaMKK inhibitor and Mg-GTP as a CaMKK-dependent phosphate donor might be useful to identify previously unidentified downstream target substrates of CaMKK.
AB - Background: Ca2+/calmodulin-dependent protein kinase kinase (CaMKK) phosphorylates and activates particular downstream protein kinases - including CaMKI, CaMKIV, and AMPK - to stimulate multiple Ca2+-signal transduction pathways. To identify previously unidentified CaMKK substrates, we used various nucleotides as phosphate donors to develop and characterize an in vitro phosphorylation assay for CaMKK. Results: Here, we found that the recombinant CaMKK isoforms were capable of utilizing Mg-GTP as a phosphate donor to phosphorylate the Thr residue in the activation-loop of CaMKIα (Thr177) and of AMPK (Thr172) in vitro. Kinetic analysis indicated that the K m values of CaMKK isoforms for GTP (400-500 μM) were significantly higher than those for ATP (∼15 μM), and a 2- to 4-fold decrease in V max was observed with GTP. We also confirmed that an ATP competitive CaMKK inhibitor, STO-609, also competes with GTP to inhibit the activities of CaMKK isoforms. In addition, to detect enhanced CaMKI phosphorylation in brain extracts with Mg-GTP and recombinant CaMKKs, we found potential CaMKK substrates of ∼45 kDa and ∼35 kDa whose Ca 2+/CaM-induced phosphorylation was inhibited by STO-609. Conclusions: These results indicated that screens that use STO-609 as a CaMKK inhibitor and Mg-GTP as a CaMKK-dependent phosphate donor might be useful to identify previously unidentified downstream target substrates of CaMKK.
KW - CaMKK
KW - Calmodulin
KW - GTP
KW - Phosphate donor
KW - Phosphorylation
UR - http://www.scopus.com/inward/record.url?scp=84870347155&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84870347155&partnerID=8YFLogxK
U2 - 10.1186/1471-2091-13-27
DO - 10.1186/1471-2091-13-27
M3 - Article
C2 - 23216827
AN - SCOPUS:84870347155
VL - 13
JO - BMC Chemical Biology
JF - BMC Chemical Biology
SN - 1471-2091
IS - 1
M1 - 27
ER -