In vitro selection of variants of herpes simplex virus type 1 which differ in cytopathic changes

Jorge Padilla, Masao Yamada, Yasushi Takahashi, Takashi Tsukazaki, Jun Nakamura, Mariko Yoshida, Fumio Uno, Yujiro Arao, Shiro Nii

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Abstract

To analyze the mechanisms for in vitro emergence of the syncytial variants of herpes simplex virus type 1 (HSV-1), several cell lines were infected with a mixture of equal amounts of two HSV-1 variants, one syncytial and the other non-syncytial, and changes in their relative abundance were monitored during passage. With a combination of two variants of the Miyama strain of HSV-1, the syncytial variant became dominant during passage in Vero, RK-13 and FL cells. On the other hand, the ratios of the two variants remained around 1:1 during the passage in HEp-2, MGC and HEL cells. In another set of variants of the SKO strain of HSV-1, the outcomes were different from those of the Miyama strain in the FL, MGC and HEp-2 cells. The ratios of the two variants remained around 1:1 during passage in FL cells, while the non-syncytial variant became dominant during passage in MGC and HEp-2 cells. In addition, we examined the effects of a complement and interferon-β (IFN-β) on the outcome of the selection. As a result, the complement slowed the selection of a syncytial variant, whereas IFN-β facilitated it.

Original languageEnglish
Pages (from-to)203-207
Number of pages5
JournalMICROBIOLOGY and IMMUNOLOGY
Volume41
Issue number3
DOIs
Publication statusPublished - 1997

Keywords

  • Cytopathic effect
  • Glycoprotein C
  • Herpes simplex virus

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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    Padilla, J., Yamada, M., Takahashi, Y., Tsukazaki, T., Nakamura, J., Yoshida, M., Uno, F., Arao, Y., & Nii, S. (1997). In vitro selection of variants of herpes simplex virus type 1 which differ in cytopathic changes. MICROBIOLOGY and IMMUNOLOGY, 41(3), 203-207. https://doi.org/10.1111/j.1348-0421.1997.tb01191.x