In vitro selection of variants of herpes simplex virus type 1 which differ in cytopathic changes

Jorge Padilla, Masao Yamada, Yasushi Takahashi, Takashi Tsukazaki, Jun Nakamura, Mariko Yoshida, Fumio Uno, Yujiro Arao, Shiro Nii

Research output: Contribution to journalArticle

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Abstract

To analyze the mechanisms for in vitro emergence of the syncytial variants of herpes simplex virus type 1 (HSV-1), several cell lines were infected with a mixture of equal amounts of two HSV-1 variants, one syncytial and the other non-syncytial, and changes in their relative abundance were monitored during passage. With a combination of two variants of the Miyama strain of HSV-1, the syncytial variant became dominant during passage in Vero, RK-13 and FL cells. On the other hand, the ratios of the two variants remained around 1:1 during the passage in HEp-2, MGC and HEL cells. In another set of variants of the SKO strain of HSV-1, the outcomes were different from those of the Miyama strain in the FL, MGC and HEp-2 cells. The ratios of the two variants remained around 1:1 during passage in FL cells, while the non-syncytial variant became dominant during passage in MGC and HEp-2 cells. In addition, we examined the effects of a complement and interferon-β (IFN-β) on the outcome of the selection. As a result, the complement slowed the selection of a syncytial variant, whereas IFN-β facilitated it.

Original languageEnglish
Pages (from-to)203-207
Number of pages5
JournalMicrobiology and Immunology
Volume41
Issue number3
Publication statusPublished - 1997

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Human Herpesvirus 1
Interferons
In Vitro Techniques
Cell Line

Keywords

  • Cytopathic effect
  • Glycoprotein C
  • Herpes simplex virus

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

Cite this

Padilla, J., Yamada, M., Takahashi, Y., Tsukazaki, T., Nakamura, J., Yoshida, M., ... Nii, S. (1997). In vitro selection of variants of herpes simplex virus type 1 which differ in cytopathic changes. Microbiology and Immunology, 41(3), 203-207.

In vitro selection of variants of herpes simplex virus type 1 which differ in cytopathic changes. / Padilla, Jorge; Yamada, Masao; Takahashi, Yasushi; Tsukazaki, Takashi; Nakamura, Jun; Yoshida, Mariko; Uno, Fumio; Arao, Yujiro; Nii, Shiro.

In: Microbiology and Immunology, Vol. 41, No. 3, 1997, p. 203-207.

Research output: Contribution to journalArticle

Padilla, J, Yamada, M, Takahashi, Y, Tsukazaki, T, Nakamura, J, Yoshida, M, Uno, F, Arao, Y & Nii, S 1997, 'In vitro selection of variants of herpes simplex virus type 1 which differ in cytopathic changes', Microbiology and Immunology, vol. 41, no. 3, pp. 203-207.
Padilla J, Yamada M, Takahashi Y, Tsukazaki T, Nakamura J, Yoshida M et al. In vitro selection of variants of herpes simplex virus type 1 which differ in cytopathic changes. Microbiology and Immunology. 1997;41(3):203-207.
Padilla, Jorge ; Yamada, Masao ; Takahashi, Yasushi ; Tsukazaki, Takashi ; Nakamura, Jun ; Yoshida, Mariko ; Uno, Fumio ; Arao, Yujiro ; Nii, Shiro. / In vitro selection of variants of herpes simplex virus type 1 which differ in cytopathic changes. In: Microbiology and Immunology. 1997 ; Vol. 41, No. 3. pp. 203-207.
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