In vitro secretion of TNF-α from bone marrow mononuclear cells incubated on amino group modified TiO2 nano-composite under ultrasound irradiation

T. Furuzono; M. Masuda; N. Nitta; A. Kaya; T. Yamane; M. Okada

doi:10.1016/j.mseb.2009.12.014

In vitro secretion of TNF-α from bone marrow mononuclear cells incubated on amino group modified TiO₂ nano-composite under ultrasound irradiation

T. Furuzono, M. Masuda, N. Nitta, A. Kaya, T. Yamane, M. Okada

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

It is recently known that titanium dioxide (TiO₂) can be excited by ultrasound and release of OH radicals on the surface. In this study, secretion of an indirect angiogenic factor, tumor necrosis factor-α (TNF-α), from bone marrow mononuclear cells (BM-MNC) incubated on amino group modified TiO₂ nano-particles covalently coated on polyester fabric (TiO₂/PET) under ultrasonic irradiation was examined in vitro. The cell viability and TNF-α secretion were measured under ultrasound irradiation condition with 255 mW/cm² of intensity, which is below the highest output (1 W/cm²) specified in the safety standard for a medical ultrasonic diagnostic apparatus. The living cell number on the TiO ₂/PET and original PET with/without continuous ultrasound irradiation was unchanged statistically by ANOVA test. TNF-α secretion level from BM-MNC remarkably increased on the TiO₂/PET under ultrasonic irradiation without cell damage. It was, therefore, thought that the high level of TNF-α secretion on the TiO₂ nano-composite by ultrasound irradiation was due to oxidative stress induced from OH radicals on TiO ₂.

Original language	English
Pages (from-to)	191-194
Number of pages	4
Journal	Materials Science and Engineering B: Solid-State Materials for Advanced Technology
Volume	173
Issue number	1-3
DOIs	https://doi.org/10.1016/j.mseb.2009.12.014
Publication status	Published - 2010
Externally published	Yes

Keywords

Angiogenic factor
Composite
Titanium dioxide
Ultrasound

ASJC Scopus subject areas

Materials Science(all)
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

Access to Document

10.1016/j.mseb.2009.12.014

Cite this

Furuzono, T., Masuda, M., Nitta, N., Kaya, A., Yamane, T., & Okada, M. (2010). In vitro secretion of TNF-α from bone marrow mononuclear cells incubated on amino group modified TiO₂ nano-composite under ultrasound irradiation. Materials Science and Engineering B: Solid-State Materials for Advanced Technology, 173(1-3), 191-194. https://doi.org/10.1016/j.mseb.2009.12.014

In vitro secretion of TNF-α from bone marrow mononuclear cells incubated on amino group modified TiO₂ nano-composite under ultrasound irradiation. / Furuzono, T.; Masuda, M.; Nitta, N. et al.

In: Materials Science and Engineering B: Solid-State Materials for Advanced Technology, Vol. 173, No. 1-3, 2010, p. 191-194.

Research output: Contribution to journal › Article › peer-review

Furuzono, T, Masuda, M, Nitta, N, Kaya, A, Yamane, T & Okada, M 2010, 'In vitro secretion of TNF-α from bone marrow mononuclear cells incubated on amino group modified TiO₂ nano-composite under ultrasound irradiation', Materials Science and Engineering B: Solid-State Materials for Advanced Technology, vol. 173, no. 1-3, pp. 191-194. https://doi.org/10.1016/j.mseb.2009.12.014

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AU - Yamane, T.

AU - Okada, M.

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