TY - JOUR
T1 - In vitro reproduction of endochondral ossification using a 3D mesenchymal stem cell construct
AU - Sasaki, Jun Ichi
AU - Matsumoto, Takuya
AU - Egusa, Hiroshi
AU - Matsusaki, Michiya
AU - Nishiguchi, Akihiro
AU - Nakano, Takayoshi
AU - Akashi, Mitsuru
AU - Imazato, Satoshi
AU - Yatani, Hirofumi
PY - 2012
Y1 - 2012
N2 - Endochondral ossification is one of the essential bone development processes in vertebrates. Although researchers from a variety of fields, including cellular/molecular biology, chemistry, and materials science, have worked to gain a better understanding of the tissue development, integration of findings from these different fields remains a major challenge. An in vitro model system that reproduces endochondral ossification would be a valuable tool for overcoming this problem, because an in vitro standardized model system can be easily accessed by researchers from different fields. Here, we fabricated a large 3D mesenchymal stem cell (MSC) construct with a ball-like morphology, which is termed a cell ball, and cultured it under a hypoxia condition, since hypoxia causes chondrogenic differentiation of MSCs in primordial cartilage, which is crucial for endochondral ossification. Region-specific chondrogenic differentiation of MSCs and mineralization within the cartilage tissue were observed in the cell ball. The precipitated minerals were detected as hydroxyapatite. Consequently, a 3D construct consisting of mineralized tissue surrounded by cartilage tissue was obtained. Moreover, the angiogenic activity of this synthesized tissue changed depending on the chondrogenic phenotype remains in the tissue, which is similar to what happens in the ossification process. Thus, this MSC cell ball system clearly reproduced the initial stage of endochondral ossification in vitro. This system is a promising tool for use as an in vitro model for investigating bone tissue development.
AB - Endochondral ossification is one of the essential bone development processes in vertebrates. Although researchers from a variety of fields, including cellular/molecular biology, chemistry, and materials science, have worked to gain a better understanding of the tissue development, integration of findings from these different fields remains a major challenge. An in vitro model system that reproduces endochondral ossification would be a valuable tool for overcoming this problem, because an in vitro standardized model system can be easily accessed by researchers from different fields. Here, we fabricated a large 3D mesenchymal stem cell (MSC) construct with a ball-like morphology, which is termed a cell ball, and cultured it under a hypoxia condition, since hypoxia causes chondrogenic differentiation of MSCs in primordial cartilage, which is crucial for endochondral ossification. Region-specific chondrogenic differentiation of MSCs and mineralization within the cartilage tissue were observed in the cell ball. The precipitated minerals were detected as hydroxyapatite. Consequently, a 3D construct consisting of mineralized tissue surrounded by cartilage tissue was obtained. Moreover, the angiogenic activity of this synthesized tissue changed depending on the chondrogenic phenotype remains in the tissue, which is similar to what happens in the ossification process. Thus, this MSC cell ball system clearly reproduced the initial stage of endochondral ossification in vitro. This system is a promising tool for use as an in vitro model for investigating bone tissue development.
UR - http://www.scopus.com/inward/record.url?scp=84874401650&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84874401650&partnerID=8YFLogxK
U2 - 10.1039/c2ib20027a
DO - 10.1039/c2ib20027a
M3 - Article
C2 - 22868898
AN - SCOPUS:84874401650
VL - 4
SP - 1207
EP - 1214
JO - Integrative Biology (United Kingdom)
JF - Integrative Biology (United Kingdom)
SN - 1757-9694
IS - 10
ER -