In vitro reproduction of endochondral ossification using a 3D mesenchymal stem cell construct.

Jun Ichi Sasaki, Takuya Matsumoto, Hiroshi Egusa, Michiya Matsusaki, Akihiro Nishiguchi, Takayoshi Nakano, Mitsuru Akashi, Satoshi Imazato, Hirofumi Yatani

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Endochondral ossification is one of the essential bone development processes in vertebrates. Although researchers from a variety of fields, including cellular/molecular biology, chemistry, and materials science, have worked to gain a better understanding of the tissue development, integration of findings from these different fields remains a major challenge. An in vitro model system that reproduces endochondral ossification would be a valuable tool for overcoming this problem, because an in vitro standardized model system can be easily accessed by researchers from different fields. Here, we fabricated a large 3D mesenchymal stem cell (MSC) construct with a ball-like morphology, which is termed a cell ball, and cultured it under a hypoxia condition, since hypoxia causes chondrogenic differentiation of MSCs in primordial cartilage, which is crucial for endochondral ossification. Region-specific chondrogenic differentiation of MSCs and mineralization within the cartilage tissue were observed in the cell ball. The precipitated minerals were detected as hydroxyapatite. Consequently, a 3D construct consisting of mineralized tissue surrounded by cartilage tissue was obtained. Moreover, the angiogenic activity of this synthesized tissue changed depending on the chondrogenic phenotype remains in the tissue, which is similar to what happens in the ossification process. Thus, this MSC cell ball system clearly reproduced the initial stage of endochondral ossification in vitro. This system is a promising tool for use as an in vitro model for investigating bone tissue development.

Original languageEnglish
Pages (from-to)1207-1214
Number of pages8
JournalIntegrative biology : quantitative biosciences from nano to macro
Volume4
Issue number10
DOIs
Publication statusPublished - Oct 2012

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Stem cells
Mesenchymal Stromal Cells
Osteogenesis
Reproduction
Tissue
Cartilage
Bone Development
Research Personnel
Bone
Cytology
Durapatite
Molecular biology
Materials science
Minerals
Cell Biology
Vertebrates
In Vitro Techniques
Molecular Biology
Cultured Cells
Phenotype

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

In vitro reproduction of endochondral ossification using a 3D mesenchymal stem cell construct. / Sasaki, Jun Ichi; Matsumoto, Takuya; Egusa, Hiroshi; Matsusaki, Michiya; Nishiguchi, Akihiro; Nakano, Takayoshi; Akashi, Mitsuru; Imazato, Satoshi; Yatani, Hirofumi.

In: Integrative biology : quantitative biosciences from nano to macro, Vol. 4, No. 10, 10.2012, p. 1207-1214.

Research output: Contribution to journalArticle

Sasaki, JI, Matsumoto, T, Egusa, H, Matsusaki, M, Nishiguchi, A, Nakano, T, Akashi, M, Imazato, S & Yatani, H 2012, 'In vitro reproduction of endochondral ossification using a 3D mesenchymal stem cell construct.', Integrative biology : quantitative biosciences from nano to macro, vol. 4, no. 10, pp. 1207-1214. https://doi.org/10.1039/c2ib20027a
Sasaki, Jun Ichi ; Matsumoto, Takuya ; Egusa, Hiroshi ; Matsusaki, Michiya ; Nishiguchi, Akihiro ; Nakano, Takayoshi ; Akashi, Mitsuru ; Imazato, Satoshi ; Yatani, Hirofumi. / In vitro reproduction of endochondral ossification using a 3D mesenchymal stem cell construct. In: Integrative biology : quantitative biosciences from nano to macro. 2012 ; Vol. 4, No. 10. pp. 1207-1214.
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