In vitro penetration of zona pellucida of salt-stored bovine oocytes before and after maturation by frozen-thawed spermatozoa

R. C. Chian, K. Niwa, K. Okuda

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Bovine oocytes, before and after maturation in culture, were stored in PBS with 2 M-(NH4)2SO4 + 0.1% dextran or 2 M-(NH4)2SO4 + 40 mM-Hepes + 0.5% dextran and were inseminated with frozen-thawed spermatozoa in BO medium with caffeine (5 mM) and heparin (10 μg/ml). The penetration rates of mature oocytes were very low (19 to 24%) and not significantly different between the two salt solutions in which the oocytes were stored for 2 to 89 days. Significantly lower (P < 0.01) penetration rates were observed in immature (7 to 8%) than in mature (20 to 21%) oocytes stored in the two solutions. The synergistic effect of caffeine and heparin was observed in the penetration rate of fresh mature oocytes but not in the stored oocytes, indicating the difficulty of assessing sperm capacitation and/or acrosome reaction of salt-stored mature bovine oocytes under the present condition. Using 0.1% protease the solubility of the zonae decreased in salt-stored but not in fresh oocytes, but there was no significant difference between the immature and mature oocytes regardless of storage in the salt solutions. It appears from these results that some alteration was induced in the nature of zona glycoprotein by ammonium sulfate solution.

Original languageEnglish
Pages (from-to)209-219
Number of pages11
JournalTheriogenology
Volume36
Issue number2
DOIs
Publication statusPublished - Aug 1991

Keywords

  • cattle
  • oocyte
  • salt-storage
  • sperm fertility
  • zonae

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

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