In-vitro penetration of pig oocytes matured in culture by frozen-thawed ejaculated spermatozoa

W. H. Wang, K. Niwa, K. Okuda

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Pig oocytes matured in culture were inseminated with frozen-thawed ejaculated spermatozoa without preincubation in modified tissue culture medium (TCM) 199. High penetration rates (85-89%) and increased incidence of polyspermy were obtained at 25-100 x 106 spermatozoa/ml. Wide variation in penetration rates (16-89%) was observed in oocytes inseminated in medium containing 5mM caffeine and at 25-50 x 106 spermatozoa/ml obtained from 6 boars, regardless of sperm motility. At 25-50 x 106 spermatozoa/ml, penetration rates of oocytes were dependent upon the concentration of caffeine in the medium: there was no penetration without caffeine, but penetration was highest (89%) with 5mM caffeine. None of the oocytes was penetrated in the medium supplemented with heparin at 5-40 μg/ml. When heparin was included in the medium with 5mM caffeine, it inhibited the efficacy of caffeine to promote sperm penetration of oocytes.

Original languageEnglish
Pages (from-to)491-496
Number of pages6
JournalJournal of Reproduction and Fertility
Volume93
Issue number2
Publication statusPublished - 1991

Fingerprint

Caffeine
Oocytes
Spermatozoa
Swine
Heparin
Sperm-Ovum Interactions
Sperm Motility
Culture Media
In Vitro Techniques
Incidence

Keywords

  • Frozen ejaculated sperm
  • In-vitro fertilization
  • Oocyte
  • Pig

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Physiology
  • Embryology
  • Obstetrics and Gynaecology

Cite this

In-vitro penetration of pig oocytes matured in culture by frozen-thawed ejaculated spermatozoa. / Wang, W. H.; Niwa, K.; Okuda, K.

In: Journal of Reproduction and Fertility, Vol. 93, No. 2, 1991, p. 491-496.

Research output: Contribution to journalArticle

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