In vitro odor-aversion conditioning in a terrestrial mollusk

Tsuyoshi Inoue, Masayoshi Murakami, Satoshi Watanabe, Yasuko Inokuma, Yutaka Kirino

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We developed an in vitro odor-aversion conditioning system in the terrestrial mollusk, Limax, and found a behavioral correlate of network oscillation in the olfactory CNS. We first examined the odor-induced behavior of Limax, after odor-aversion conditioning in vivo. Shortening of mantle muscles was specifically observed in response to aversively conditioned odors. We previously identified that parietal nerves, which project to the mantle muscle in Limax, regulate shortening of the mantle muscle. We therefore isolated whole brains containing noses (sensory organs) and parietal nerves (motor output), and applied an odor-aversion conditioning paradigm to these in vitro preparations. Before the in vitro conditioning, application of attractive odors to the noses did not elicit any discharge in the parietal nerves. However, after odor-aversion conditioning, discharges in the parietal nerves were observed in response to the natively attractive but aversively conditioned odors. We also found that network oscillation frequency in the procerebrum (PC), the olfactory CNS of Limax, increased specifically in response to the aversively conditioned odors that elicited avoidance behavior. In naive (nonconditioned) preparations, increases in the PC oscillation frequency were observed specifically in response to innately aversive odors. These results indicate that the isolated brains have an ability of odor learning. They also suggest that changes in PC network oscillation are associated with aversively conditioned and innately aversive odors, both of which elicit avoidance behavior. This in vitro conditioning system would be an effective approach for exploring the neural mechanism to determine the aversion to odors.

Original languageEnglish
Pages (from-to)3898-3903
Number of pages6
JournalJournal of Neurophysiology
Volume95
Issue number6
DOIs
Publication statusPublished - Jun 1 2006
Externally publishedYes

Fingerprint

Mollusca
Avoidance Learning
Nose
Muscles
Odorants
In Vitro Techniques
Conditioning (Psychology)
Aptitude
Brain

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

Cite this

In vitro odor-aversion conditioning in a terrestrial mollusk. / Inoue, Tsuyoshi; Murakami, Masayoshi; Watanabe, Satoshi; Inokuma, Yasuko; Kirino, Yutaka.

In: Journal of Neurophysiology, Vol. 95, No. 6, 01.06.2006, p. 3898-3903.

Research output: Contribution to journalArticle

Inoue, T, Murakami, M, Watanabe, S, Inokuma, Y & Kirino, Y 2006, 'In vitro odor-aversion conditioning in a terrestrial mollusk', Journal of Neurophysiology, vol. 95, no. 6, pp. 3898-3903. https://doi.org/10.1152/jn.00853.2005
Inoue, Tsuyoshi ; Murakami, Masayoshi ; Watanabe, Satoshi ; Inokuma, Yasuko ; Kirino, Yutaka. / In vitro odor-aversion conditioning in a terrestrial mollusk. In: Journal of Neurophysiology. 2006 ; Vol. 95, No. 6. pp. 3898-3903.
@article{4dcf10d8760740d786e4ac2f4d8b3a93,
title = "In vitro odor-aversion conditioning in a terrestrial mollusk",
abstract = "We developed an in vitro odor-aversion conditioning system in the terrestrial mollusk, Limax, and found a behavioral correlate of network oscillation in the olfactory CNS. We first examined the odor-induced behavior of Limax, after odor-aversion conditioning in vivo. Shortening of mantle muscles was specifically observed in response to aversively conditioned odors. We previously identified that parietal nerves, which project to the mantle muscle in Limax, regulate shortening of the mantle muscle. We therefore isolated whole brains containing noses (sensory organs) and parietal nerves (motor output), and applied an odor-aversion conditioning paradigm to these in vitro preparations. Before the in vitro conditioning, application of attractive odors to the noses did not elicit any discharge in the parietal nerves. However, after odor-aversion conditioning, discharges in the parietal nerves were observed in response to the natively attractive but aversively conditioned odors. We also found that network oscillation frequency in the procerebrum (PC), the olfactory CNS of Limax, increased specifically in response to the aversively conditioned odors that elicited avoidance behavior. In naive (nonconditioned) preparations, increases in the PC oscillation frequency were observed specifically in response to innately aversive odors. These results indicate that the isolated brains have an ability of odor learning. They also suggest that changes in PC network oscillation are associated with aversively conditioned and innately aversive odors, both of which elicit avoidance behavior. This in vitro conditioning system would be an effective approach for exploring the neural mechanism to determine the aversion to odors.",
author = "Tsuyoshi Inoue and Masayoshi Murakami and Satoshi Watanabe and Yasuko Inokuma and Yutaka Kirino",
year = "2006",
month = "6",
day = "1",
doi = "10.1152/jn.00853.2005",
language = "English",
volume = "95",
pages = "3898--3903",
journal = "Journal of Neurophysiology",
issn = "0022-3077",
publisher = "American Physiological Society",
number = "6",

}

TY - JOUR

T1 - In vitro odor-aversion conditioning in a terrestrial mollusk

AU - Inoue, Tsuyoshi

AU - Murakami, Masayoshi

AU - Watanabe, Satoshi

AU - Inokuma, Yasuko

AU - Kirino, Yutaka

PY - 2006/6/1

Y1 - 2006/6/1

N2 - We developed an in vitro odor-aversion conditioning system in the terrestrial mollusk, Limax, and found a behavioral correlate of network oscillation in the olfactory CNS. We first examined the odor-induced behavior of Limax, after odor-aversion conditioning in vivo. Shortening of mantle muscles was specifically observed in response to aversively conditioned odors. We previously identified that parietal nerves, which project to the mantle muscle in Limax, regulate shortening of the mantle muscle. We therefore isolated whole brains containing noses (sensory organs) and parietal nerves (motor output), and applied an odor-aversion conditioning paradigm to these in vitro preparations. Before the in vitro conditioning, application of attractive odors to the noses did not elicit any discharge in the parietal nerves. However, after odor-aversion conditioning, discharges in the parietal nerves were observed in response to the natively attractive but aversively conditioned odors. We also found that network oscillation frequency in the procerebrum (PC), the olfactory CNS of Limax, increased specifically in response to the aversively conditioned odors that elicited avoidance behavior. In naive (nonconditioned) preparations, increases in the PC oscillation frequency were observed specifically in response to innately aversive odors. These results indicate that the isolated brains have an ability of odor learning. They also suggest that changes in PC network oscillation are associated with aversively conditioned and innately aversive odors, both of which elicit avoidance behavior. This in vitro conditioning system would be an effective approach for exploring the neural mechanism to determine the aversion to odors.

AB - We developed an in vitro odor-aversion conditioning system in the terrestrial mollusk, Limax, and found a behavioral correlate of network oscillation in the olfactory CNS. We first examined the odor-induced behavior of Limax, after odor-aversion conditioning in vivo. Shortening of mantle muscles was specifically observed in response to aversively conditioned odors. We previously identified that parietal nerves, which project to the mantle muscle in Limax, regulate shortening of the mantle muscle. We therefore isolated whole brains containing noses (sensory organs) and parietal nerves (motor output), and applied an odor-aversion conditioning paradigm to these in vitro preparations. Before the in vitro conditioning, application of attractive odors to the noses did not elicit any discharge in the parietal nerves. However, after odor-aversion conditioning, discharges in the parietal nerves were observed in response to the natively attractive but aversively conditioned odors. We also found that network oscillation frequency in the procerebrum (PC), the olfactory CNS of Limax, increased specifically in response to the aversively conditioned odors that elicited avoidance behavior. In naive (nonconditioned) preparations, increases in the PC oscillation frequency were observed specifically in response to innately aversive odors. These results indicate that the isolated brains have an ability of odor learning. They also suggest that changes in PC network oscillation are associated with aversively conditioned and innately aversive odors, both of which elicit avoidance behavior. This in vitro conditioning system would be an effective approach for exploring the neural mechanism to determine the aversion to odors.

UR - http://www.scopus.com/inward/record.url?scp=33744921368&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744921368&partnerID=8YFLogxK

U2 - 10.1152/jn.00853.2005

DO - 10.1152/jn.00853.2005

M3 - Article

C2 - 16495363

AN - SCOPUS:33744921368

VL - 95

SP - 3898

EP - 3903

JO - Journal of Neurophysiology

JF - Journal of Neurophysiology

SN - 0022-3077

IS - 6

ER -