Abstract
We investigated the localization and density of atrial natriuretic peptide (ANP) receptors in human renal biopsy specimens by using in vitro micro- autoradiography (ARG) of [125I]-α-human (1-28) ANP. In a preliminary study, we measured the effect of storing tissue samples on ANP binding, using in vitro micro-ARG of Wistar rat kidney under optimal conditions. Duration of the preservation period did not affect ANP binding to renal tissue until samples had been stored at -30°C for two years. A total of 11 human renal tissues were used to assay binding of ANP-ARG, including normal tissue obtained after nephrectomy because of renal cancer. ANP binding occurred predominantly within the glomerulus and, to a lesser extent, in the tubular region both in rat kidney sections and in human renal biopsy specimens. The density of ANP binding, calculated by counting grains in fixed areas, was compared with normal and pathological tissues. The density of grains tended to decrease in patients with renal dysfunction and hypertension except for one case of IgA nephropathy with normal renal function and blood pressure. The density of grains increased in a patient with nephrogenic diabetes insipidus. In the present study, we have established a method that uses in vitro micro-ARG for assessing ANP binding in human biopsy specimens.
| Original language | English |
|---|---|
| Pages (from-to) | 203-217 |
| Number of pages | 15 |
| Journal | Journal of Medicine |
| Volume | 25 |
| Issue number | 3-4 |
| Publication status | Published - 1994 |
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- Medicine(all)
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In vitro micro-autoradiography of atrial natriuretic peptide in biopsy specimens from patients with renal diseases. / Ogura, T.; Asano, N.; Katayama, E.; Oishi, T.; Mimura, Yukari; Hironaka, K.; Kashihara, N.; Makino, Hirofumi; Ota, Z.; Ogawa, N.
In: Journal of Medicine, Vol. 25, No. 3-4, 1994, p. 203-217.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro micro-autoradiography of atrial natriuretic peptide in biopsy specimens from patients with renal diseases
AU - Ogura, T.
AU - Asano, N.
AU - Katayama, E.
AU - Oishi, T.
AU - Mimura, Yukari
AU - Hironaka, K.
AU - Kashihara, N.
AU - Makino, Hirofumi
AU - Ota, Z.
AU - Ogawa, N.
PY - 1994
Y1 - 1994
N2 - We investigated the localization and density of atrial natriuretic peptide (ANP) receptors in human renal biopsy specimens by using in vitro micro- autoradiography (ARG) of [125I]-α-human (1-28) ANP. In a preliminary study, we measured the effect of storing tissue samples on ANP binding, using in vitro micro-ARG of Wistar rat kidney under optimal conditions. Duration of the preservation period did not affect ANP binding to renal tissue until samples had been stored at -30°C for two years. A total of 11 human renal tissues were used to assay binding of ANP-ARG, including normal tissue obtained after nephrectomy because of renal cancer. ANP binding occurred predominantly within the glomerulus and, to a lesser extent, in the tubular region both in rat kidney sections and in human renal biopsy specimens. The density of ANP binding, calculated by counting grains in fixed areas, was compared with normal and pathological tissues. The density of grains tended to decrease in patients with renal dysfunction and hypertension except for one case of IgA nephropathy with normal renal function and blood pressure. The density of grains increased in a patient with nephrogenic diabetes insipidus. In the present study, we have established a method that uses in vitro micro-ARG for assessing ANP binding in human biopsy specimens.
AB - We investigated the localization and density of atrial natriuretic peptide (ANP) receptors in human renal biopsy specimens by using in vitro micro- autoradiography (ARG) of [125I]-α-human (1-28) ANP. In a preliminary study, we measured the effect of storing tissue samples on ANP binding, using in vitro micro-ARG of Wistar rat kidney under optimal conditions. Duration of the preservation period did not affect ANP binding to renal tissue until samples had been stored at -30°C for two years. A total of 11 human renal tissues were used to assay binding of ANP-ARG, including normal tissue obtained after nephrectomy because of renal cancer. ANP binding occurred predominantly within the glomerulus and, to a lesser extent, in the tubular region both in rat kidney sections and in human renal biopsy specimens. The density of ANP binding, calculated by counting grains in fixed areas, was compared with normal and pathological tissues. The density of grains tended to decrease in patients with renal dysfunction and hypertension except for one case of IgA nephropathy with normal renal function and blood pressure. The density of grains increased in a patient with nephrogenic diabetes insipidus. In the present study, we have established a method that uses in vitro micro-ARG for assessing ANP binding in human biopsy specimens.
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UR - http://www.scopus.com/inward/citedby.url?scp=0028048254&partnerID=8YFLogxK
M3 - Article
C2 - 7996064
AN - SCOPUS:0028048254
VL - 25
SP - 203
EP - 217
JO - Journal of Medicine
JF - Journal of Medicine
SN - 0025-7850
IS - 3-4
ER -